cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pFB Dual6P-1 TPL2 30-397
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST TPL2 30-397
• Genbank: P41279
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAAATCTTTATGCAAGTGAAGAGCCAGCAGTTTATGAACC
CAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCTA
AGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGA
TATGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACAC
TGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAA
ACATGGTCATCACTCCCCAAAATGGACGTTACCAAATAGATTCCGATGTT
CTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGTTCTGATTTTAT
TCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCTCAAGATATAAAGACGA
AGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCT
GATGTGGAAATTCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTA
TGGCGCAGTCCTGTGGGGTGAAACTGTCCATCTCTTTATGGAAGCAGGCG
AGGGAGGGTCTGTTCTGGAGAAACTGGAGAGCTGTGGACCAATGAGAGAA
TTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGACTTGATTTTCT
ACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTTT
TCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATG
ACCGAAGATGTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACAT
GAGCCCAGAGGTCATCCTGTGCAGGGGCCATTCAACCAAAGCAGACATCT
ACAGCCTGGGGGCCACGCTCATCCACATGCAGACGGGCACCCCACCCTGG
GTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTGTACATAATCCA
CAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGGA
TGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCA
AGAGCCGCAGACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGA
TCAGCCACGCTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMENLYASEEPAVYEPSLMT
MCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHF
YGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGA
FGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVL
WGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKK
VIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEV
ILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAP
PLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPPREDQPR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15339
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal GST tag.; ; Estimated MW of expressed protein : 68626 Daltons