cDNA Clone SIK (mouse)

• Plasmid: pCMV-HA-1 SIK (mouse)
• Parent Plasmid: pCMV5 HA1
• Expressed: HA SIK
• Genbank: NM_010831
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGGTGATCATGTCGGAGTTCAGTGCGGTCCCCTCAGGCACTGG
CCAGGGCCAGCAGAAGCCCCTCCGGGTGGGCTTTTACGACGTGGAACGGA
CCCTGGGCAAAGGCAATTTTGCAGTGGTTAAGCTGGCGCGGCACCGAGTC
ACCAAAACGCAGGTTGCAATAAAAATAATTGACAAGACACGGTTAGATTC
TAGCAATCTGGAGAAGATCTACCGGGAGGTCCAGCTCATGAAACTTTTGA
ACCACCCAAATATCATCAAGCTTTATCAGGTTATGGAGACAAAGGATATG
CTCTACATTGTCACAGAATTTGCAAAAAATGGAGAAATGTTTGATTATCT
GACTTCCAACGGGCACTTGAGTGAAAACGAGGCTAGGCAGAAGTTCTGGC
AGATTCTGTCAGCCGTGGAGTACTGCCACAACCACCACATTGTCCACCGG
GACCTCAAGACGGAGAACCTGCTCCTGGACAGCAACATGGATATCAAGCT
AGCAGATTTTGGATTTGGGAATTTCTACAAGCCAGGCGAGCCTCTGTCCA
CGTGGTGTGGGAGCCCCCCGTATGCAGCCCCTGAAGTCTTCGAGGGGAAG
GAGTACGAGGGTCCCCAGCTGGACGTCTGGAGCCTCGGTGTGGTGCTGTA
CGTCCTGGTCTGTGGCTCCCTCCCCTTCGACGGGCCCAACCTGCCTACGC
TGAGACAGCGGGTACTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCT
CAAGACTGTGAGACGCTGATCAGACGCATGCTGGTCGTGGACCCCGCTAA
GCGCATCACCATAGCCCAGATCCGCCAGCACCGGTGGATGCAGGCCGACC
CCACTCTCCTGCAGCAGGATGACCCTGCCTTCGACATGCAAGGCTACACC
TCCAACCTGGGCGACTACAACGAACAGGTGCTAGGGATCATGCAGGCCCT
CGGCATCGACCGGCAGCGGACTATAGAGTCTCTGCAGAACAGCAGCTACA
ACCACTTTGCCGCCATTTACTACCTCCTACTCGAGCGCCTCAAGGAGCAT
CGAAGCGCCCAGCCCTCATCCCGGCCCACCCCTGCACCCACCAGACAGCC
CCAGCTCCGAAGCTCAGACCTCAGCAGTCTGGAGGTTCCTCAAGAAATTC
TCCCGTGTGACCCTTTCCGGCCCTCTCTGCTGTGCCCACAGCCCCAGGCC
TTGGCTCAGTCTGTCCTGCAGGCTGAGATAGACTGTGATCTCCACAGCTC
ACTTCAGCCCTTATTATTCCCCCTGGATACCAACTGCAGTGGAGTGTTCC
GGCACCGATCCATCTCCCCCAGCAGTCTGCTGGACACAGCTATCAGCGAG
GAGGCCAGGCAGGGTCCCAGCCTAGAGGAGGAACAGGAGGTCCAGGAACC
CCTGCCCGGAAGCACAGGCCGGAGGCACACATTGGCTGAAGTCTCCACCC
ATTTCTCCCCGCTCAACCCTCCTTGCATAATTGTCTCCTCCTCTGCCACG
GCGAGTCCCTCGGAAGGGACCAGCTCCGACAGCTGCCTCCCCTTCTCTGC
AAGTGAAGGTCCTGCAGGGCTTGGCAGTGGCCTGGCCACCCCAGGGCTGC
TGGGCACCAGCTCTCCAGTCAGATTGGCCTCGCCCTTCCTGGGATCACAG
TCAGCCACCCCTGTGCTCCAGACTCAGGCAGGTCTGGGCACAGCCGTCTT
ACCTCCCGTCAGCTTCCAGGAGGGACGGAGAGCGTCTGATACCTCTCTCA
CTCAGGGGCTGAAGGCCTTCCGGCAGCAGCTGAGGAAAAATGCGAGGACC
AAGGGGTTCCTGGGACTGAACAAGATCAAAGGGTTGGCTCGCCAGGTGTG
CCAGTCCTCCGTCCGAACTCCCCGGGGAGGGATGAGTACCTTCCACACCC
CAGCCCCAAGCTCCGGCCTGCAAGGCTGCACGACCAGCAATCGGGAGGGC
AGGAGCCTACTTGAAGAGGTCCTGCACCAGCAGAGGCTGCTCCAGTTACA
GCACCACTCTTCTACCGCCGCCGCCTCATCTGGCTGCCAGCAGGGCCCCC
AGCTGTCCCCGGTCCCCTATGTGCTTGCTCCCTGTGACAGCCTCCTCGTG
TCTGGAATTCCACTGCTGCCAACACCCCTTCTCCAGGCTGGCATGTCCCC
TGTGGCGTCTGCTGCGCATCTCCTGGATACCCACTTGCACATCAGTGCTG
GCCCAGTGGCCCTCCCCACTGGGCCCCTGCCACAGTGCCTCACCAGGCTG
TCCCCAGGCTGTGATCCAGCTGGGCTGCCACAGGGGGACTGTGAGATGGA
GGACCTGACCTCTGGCCAGCGGGGGACATTCGTCCTGGTACAGTGAGCGG
CCGC

Amino Acid Sequence

MYPYDVPDYAGSMVIMSEFSAVPSGTGQGQQKPLRVGFYDVERTLGKGNF
AVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPNIIK
LYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARQKFWQILSAVE
YCHNHHIVHRDLKTENLLLDSNMDIKLADFGFGNFYKPGEPLSTWCGSPP
YAAPEVFEGKEYEGPQLDVWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLE
GRFRIPFFMSQDCETLIRRMLVVDPAKRITIAQIRQHRWMQADPTLLQQD
DPAFDMQGYTSNLGDYNEQVLGIMQALGIDRQRTIESLQNSSYNHFAAIY
YLLLERLKEHRSAQPSSRPTPAPTRQPQLRSSDLSSLEVPQEILPCDPFR
PSLLCPQPQALAQSVLQAEIDCDLHSSLQPLLFPLDTNCSGVFRHRSISP
SSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNP
PCIIVSSSATASPSEGTSSDSCLPFSASEGPAGLGSGLATPGLLGTSSPV
RLASPFLGSQSATPVLQTQAGLGTAVLPPVSFQEGRRASDTSLTQGLKAF
RQQLRKNARTKGFLGLNKIKGLARQVCQSSVRTPRGGMSTFHTPAPSSGL
QGCTTSNREGRSLLEEVLHQQRLLQLQHHSSTAAASSGCQQGPQLSPVPY
VLAPCDSLLVSGIPLLPTPLLQAGMSPVASAAHLLDTHLHISAGPVALPT
GPLPQCLTRLSPGCDPAGLPQGDCEMEDLTSGQRGTFVLVQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15008
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 86471 Daltons