cDNA Clone Pellino1

• Plasmid: pGEX6P-1-Pellino1 S76A T80A S82A T86A T127A T288A GS/GA
• Parent Plasmid: pGEX6P1
• Expressed: GST-Pellino1 S76A T80A S82A T86A T127A T288A GS/GA
• Genbank: Q96FA3
• Tag: GST
• Species: Human

Sequence of Insert

GGAGCCATGTTTTCTCCTGATCAAGAAAATCATCCATTTAAAGCACCAGT
AAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATG
GCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAG
GCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGC
TGCAAAGGCAATAAGCAACAAAGACCAGCATGCCATATCATATGCTTTAG
CTCGGGCCCAGGCTGTGGTGGTTGAATATACTCATGACAGCAACACAGAT
ATGTTTCAGATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAAC
TGACACGGTTCCTGGAAGTCAAAGTAATTCTGATGCACAGTCAGTACAAA
GCACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCC
TTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTT
TCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATG
GCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACA
GAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGT
ATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGG
AAATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGT
GCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGT
GAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGT
GCCCTGTAGGGTTCAACGCACTAGCATTTCCTAGTATGAAGAGGAAAGAC
GTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACA
TGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTG
AATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGA
TGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAG
CCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGA
TCCCACTTCCTCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGT
GCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGG
ACCTCTAGACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGAMFSPDQENHPFKAPVKYGE
LIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAI
SNKDQHAISYALARAQAVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVP
GSQSNSDAQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEK
AAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLR
ETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLE
ALRQEINAARPQCPVGFNALAFPSMKRKDVVDEKQPWVYLNCGHVHGYHN
WGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGH
VCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12893
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 72999 Daltons