cDNA Clone UBCH7

• Synonyms: E2-F1; L-UBC; UBE2L3; UbcM4
• Plasmid: pET156P UBCH7
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C UBCH7
• Genbank: NM_003347.4
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCG
CAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATT
TATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAG
GGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACC
ACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAA
AGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCA
ACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCC
CCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGG
ACCGTAAAAAATTCTGTAAGAATGCTGAAGAGTTTACAAAGAAATATGGG
GAAAAGCGACCTGTGGACTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAASRRLMKELEEIRKCGMKNFRNIQV
DEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHP
NIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAE
EYSKDRKKFCKNAEEFTKKYGEKRPVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET156P UBCH7189 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12798
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 20305 Daltons.; ; Compared to reference sequence there is a silent change at E141.
• Low Temp: No