cDNA Clone UBCH8

• Synonyms: ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1, UBE2L6
• Plasmid: pET156P UBCH8
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBCH8
• Genbank: NM_004223.5 → NP_004214.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGATGGCGAGCATGCGAGTGGTGAAGGAGCTGGAGGATCTTCA
GAAGAAGCCTCCCCCATACCTGCGGAACCTGTCCAGCGATGATGCCAATG
TCCTGGTGTGGCACGCTCTCCTCCTACCCGACCAACCTCCCTACCACCTG
AAAGCCTTCAACCTGCGCATCAGCTTCCCGCCGGAGTATCCGTTCAAGCC
TCCCATGATCAAATTCACAACCAAGATCTACCACCCCAACGTGGACGAGA
ACGGACAGATTTGCCTGCCCATCATCAGCAGTGAGAACTGGAAGCCTTGC
ACCAAGACTTGCCAAGTCCTGGAGGCCCTCAATGTGCTGGTGAATAGACC
GAATATCAGGGAGCCCCTGCGGATGGACCTCGCTGACCTGCTGACACAGA
ATCCGGAGCTGTTCAGAAAGAATGCCGAAGAGTTCACCCTCCGATTCGGA
GTGGACCGGCCCTCCTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMMASMRVVKELEDLQKKPPPYLRNLSS
DDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHP
NVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLAD
LLTQNPELFRKNAEEFTLRFGVDRPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET156P UBCH846.25 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12797
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for bacterial expression. Isoform 1; ; Estimated MW of expressed protein : 20212 Daltons
• Low Temp: No