cDNA Clone USP20

• Plasmid: pcDNA5-FRT/TO/TAP USP20
• Parent Plasmid: pCDNA5 FRT/TO TAP
• Expressed: TAP USP20
• Genbank: AAH39593.1
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGG
AGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGGGAACCTGTCAGT
CGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCTGC
CCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGC
ACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGT
GGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCC
CCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACC
CTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGT
CTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGAAG
AACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAA
TTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCA
CAGATAAGAAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAG
GTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCCACCAGTCTGTCTCA
TGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACA
CCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAG
GAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTC
GAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATG
AGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAG
GGGCGTGGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGA
TGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGAAGGACCGCAAGT
TCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCT
GATGTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCC
CCCGTCACCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATG
ATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCACCAC
GAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAG
GATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCA
GCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTT
GACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATC
CACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGG
AGGACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAG
CCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCTTCAT
TGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTT
GGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCT
GATGAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCT
GCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGT
GCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATC
AACAGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCT
TGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTCTCGGTCA
TCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAG
AACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGA
AGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACA
GGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTG
GCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTG
GCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGA
CCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACATC
GACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAA
CAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCT
GCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGAC
ACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGT
CATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCA
AGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCA
CAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGG
GCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTG
GCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAG
AACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTGTGAGC
GGCCGC

Amino Acid Sequence

MDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQS
ANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQS
LKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRR
WKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSMGDSRDLCPH
LDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADH
STIHAQAKKHNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQ
DSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAAL
QALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVP
TSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEA
RDSDSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETE
LLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMAALDQP
AEAQPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPR
ASPVRMAPSYVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLT
CDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQG
WLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCE
RCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLD
LRPFLAKECTSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDD
QYVTEVHETVVQNAEGYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFY
VSREWLNKFNTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVW
EHLYNRFGGGPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAE
ESPGVIYCISMQWFREWEAFVKGKDNEPPGPIDNSRIAQVKGSGHVQLKQ
GADYGQISEETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAET
RAV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12766
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter TAP for mammalian expression. ; ; Estimated MW of expressed protein : 123208 Daltons