cDNA Clone PPP1R3F

• Plasmid: pEBG6P-PPP1R3F 1-761
• Parent Plasmid: pEBG6P
• Expressed: GST PPP1R3F 1-761
• Genbank: XM_372210
• Tag: GST
• Species: Human

Sequence of Insert

GGTACCATGGCGCGTACGGCCCCTGTGGAGCCCCCGCTGCGGCATTCCGC
GCCCCCCTCGCCGGCCGCGGGTGAGCCCCGCACCTCGGTCGAGGCGGCGG
TGGCCCCGCGGAGGGTGCTGTTCGCCGACGAGGCCTTGGGGCTGCCGCTG
GCGCAGTTGCGCCGCTACCGGCCGTGGGGCGGGCCCGGGGCGGGCAAGAT
GGCGGCGGCGGCCGGGCAAGATGGCGGCGGCGGCGGCGGGGCCGACGAGG
ACGACGATGGCGAGGATGGGGATGAAGGGGAGGAGGAAGAGGAGGCTTGC
CCCGAGCCCTCACCGCTGTGCCCCGTCCCCGCTGGCGGGGGGTTTTACCT
GGTCCCCACATTTTCGCTGCCGCCCGCGCCGGGCCGTCTGGAGCGCTTGG
GGCGCGTCATGGTGGAGCTGGAGGCGCTGCTGCCGCCTCCCGGAGCGGTC
CCCGGGGGTGCCGGGGTGTGGGTGCCTGGGGGCCGCCCGCCGGTGCTGCG
CGGGTTGGTACGCGTGCTGAACCGCTCCTTCGAGAAGGCGGTGCACGTGC
GGGCCTCACACGACGGCTGGGCTTCCTTTTGCGACCACCCAGCGCGCTAC
GTCCCGCGCAGCCCGCCGTGGGCAGGAGCGGGAGGAACAGGAGCAGGAGA
TCCCATCCTGGATCCGGGGCTCGGCCTGGGTCCCGGCCAGGCATCCGCCT
CCTCGCCCGACGACGGCGGCCGCACCGACCGCTTTGCCTTCCAGCTGCCC
TTTGCTGAGGGCGCGGGCGATGGGGCGCGCCTCGACTTCGTGGTGCGCTA
TGAGACCCCTGAGGGCACTTTCTGGGCCAACAACCACGGCCGCAACTACA
CAGTCCTGCTCCGGATCGCACCCGCTCCCACACCCACTGATGCCGAAGGG
CTGCCCCAGCAGCAGCAGCTGCCGCAGCTGGAGCCACAGCCCGAGTGCCA
GGGTCCCGTGGAGGCTGAGGCCAGGCAGCTGAAGAGCTGCATGAAGCCGG
TGAGGCGCAGGCCTGCCGAGGAGGAACTGAAGACGAAGAACATGGATGAT
AACACCTTTGCCATGGCAGAGCATCCTGATGTCCAGGAGTCAGTGGGTCC
ACTGGTAGCCCCCACCCCTCTCCGTCCATGGCCCCAGATGACACTTCAGG
TTTCTGACGTTCCGATGACTGGCAACCCCGCAGAAGAAGGTGATGTCCCC
AGAAGCAGTCCACCTGTGGCTTTTACAGAGGTCCTCCAGGCACCGGCCAT
CAGGATTCCCCCCTCCTCCCCTCTCTGTGGCCTGGGTGGCTCCCCCAGAG
ACCAGGCCTCAGGGCCCGATGCGAGCGAGGGGGCCACCGGGCCTTTCCTG
GAGCCCAGTCAGCAGCAGGCAGAGGCCACATGGGGAGTATCGAGTGAGAA
TGGAGGGGGGCTGGAGGCTGTGAGTGGGTCAGAGGAGCTGCTCGGTGAGG
ACACCATCGACCAGGAGCTGGAGCAGCTCTACCTGTCTCACCTGAGCCGC
CTACGGGCTGCTGTGGCTGCGGGTGGGGCAGGGGGTGGTGGGGAGGGCTC
CACAGATGGAGGGATGTCCCCCAGCCATCCCCTGGGCATACTGACGGACC
GCGACCTGATCTTGAAGTGGCCTGGCCCTGAGCGGGCCCTGAACAGCGCC
CTGGCTGAGGAGATCACGCTGCACTATGCCCGGCTGGGGCGTGGCGTGGA
GCTCATCAAGGACACCGAAGACCCTGATGATGAAGGGGAGGGTGAAGAGG
GGCTCTCTGTCACACCCTCCAGCCCAGAAGGGGACAGCCCCAAGGAATCG
CCTCCAGAAATCCTCTCCGGGGCCCGTTCTGTGGTAGCCACGATGGGAGA
TGTGTGGCTCCCATGGGCAGAGGGCTCAGGATGTGACGGCCCTGTGGTTC
TGGGTACAGAGGGTCAGTTCATTGGGGATCCTGAGAAAGGGATGGGCAAG
GACACCAGCTCTTTGCACATGAATAGGGTGATAGCTGGGGTGACTGAGTC
CCTGGGGGAGGCCGGGACAGAAGCCCAGATAGAGGTCACCAGTGAGTGGG
CAGGCAGCTTGGATCCCATATCTGGCAAGGAGCCAGCCTCTCCCGTCCTT
CTGCAGGGGCAAAATCCCACCCTCCTCAGTCCCTTGGGGGCCGAAGTCTG
TCTCTCTAGTGTAGCCAGGCCTCATGTGAGCTCCCAGGATGAAAAGGATG
CAGGCCCAAGCCTTGAACCCCCAAAGAAGTCTCCCACCCTAGCAGTCCCT
GCAGAATGTGTGTGTGCACTGCCTCCTCAGCTCCGGGGGTAATTGACCCA
GACTCTGGGGGTCCTGGCCGGGCTAGTGGTGGTCCCTGTGGCTCTGAACA
GCGGTGTGTCCCTCCTGGTGCTTGCGCTGTGCCTCTCTCTGGCTTGGTTC
TCATAGGGTACC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMARTAPVEP
PLRHSAPPSPAAGEPRTSVEAAVAPRRVLFADEALGLPLAQLRRYRPWGG
PGAGKMAAAAGQDGGGGGGADEDDDGEDGDEGEEEEEACPEPSPLCPVPA
GGGFYLVPTFSLPPAPGRLERLGRVMVELEALLPPPGAVPGGAGVWVPGG
RPPVLRGLVRVLNRSFEKAVHVRASHDGWASFCDHPARYVPRSPPWAGAG
GTGAGDPILDPGLGLGPGQASASSPDDGGRTDRFAFQLPFAEGAGDGARL
DFVVRYETPEGTFWANNHGRNYTVLLRIAPAPTPTDAEGLPQQQQLPQLE
PQPECQGPVEAEARQLKSCMKPVRRRPAEEELKTKNMDDNTFAMAEHPDV
QESVGPLVAPTPLRPWPQMTLQVSDVPMTGNPAEEGDVPRSSPPVAFTEV
LQAPAIRIPPSSPLCGLGGSPRDQASGPDASEGATGPFLEPSQQQAEATW
GVSSENGGGLEAVSGSEELLGEDTIDQELEQLYLSHLSRLRAAVAAGGAG
GGGEGSTDGGMSPSHPLGILTDRDLILKWPGPERALNSALAEEITLHYAR
LGRGVELIKDTEDPDDEGEGEEGLSVTPSSPEGDSPKESPPEILSGARSV
VATMGDVWLPWAEGSGCDGPVVLGTEGQFIGDPEKGMGKDTSSLHMNRVI
AGVTESLGEAGTEAQIEVTSEWAGSLDPISGKEPASPVLLQGQNPTLLSP
LGAEVCLSSVARPHVSSQDEKDAGPSLEPPKKSPTLAVPAECVCALPPQL
RG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12623
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; ; Estimated MW of expressed protein : 106739 Daltons