cDNA Clone Pellino2

• Plasmid: pCMV-HA-1 Pellino2 S78A T82A T88A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA Pellino2 S78A T82A T88A
• Genbank: AAH09476.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGA
GCCAGTGAAATACGGGGAGCTGGTGGTGCTCGGGTACAATGGTGCTTTAC
CCAATGGAGATAGAGGACGGAGGAAAAGTAGATTTGCCCTCTACAAGCGG
CCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCATGTGATATCCACGCC
CCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACGCTATATCCTACG
CTTTGTCAAGGAATCAGGCTGTGGTGGTGGAGTACACACATGATAAGGAT
ACGGATATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGT
TGTCACAGACACGATTTCTGGCAGCCAGAACACGGACGAAGCCCAGATCA
CACAGAGCACCATATCCAGGTTCGCCTGCAGGATCGTGTGCGACAGGAAT
GAACCTTACACAGCACGGATATTCGCCGCCGGATTTGACTCTTCCAAAAA
CATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGCCACA
TGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGC
TTCACCGAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGG
AGATGTGTACACCTTGCGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGC
TGGTGGAAAGTGAGACCAACGTCCTGCAGGACGGCTCCCTCATTGACCTG
TGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGGCTTTTTCATACTCC
AACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCCCGGC
CTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGG
AAAGAGGTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCA
CGTGCACGGGTACCACAACTGGGGCCATCGGAGTGACACGGAGGCCAACG
AGAGGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTATGTGCCTCTCTGG
CTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGACCGCCAACTCATGC
TTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATACTGGT
CTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCT
TTCTGTGCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTT
CCAAGGTCCAATTGACTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMFSPGQEEHCAPNKEPVKYGELVVLGYNGALPNGDRGR
RKSRFALYKRPKANGVKPSTVHVISTPQASKAISCKGQHAISYALSRNQA
VVVEYTHDKDTDMFQVGRSTESPIDFVVTDTISGSQNTDEAQITQSTISR
FACRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDGHMDGLTTN
GVLVMHPRGGFTEESQPGVWREISVCGDVYTLRETRSAQQRGKLVESETN
VLQDGSLIDLCGATLLWRTADGLFHTPTQKHIEALRQEINAARPQCPVGL
NTLAFPSINRKEVVEEKQPWAYLSCGHVHGYHNWGHRSDTEANERECPMC
RTVGPYVPLWLGCEAGFYVDAGPPTHAFTPCGHVCSEKSAKYWSQIPLPH
GTHAFHAACPFCATQLVGEQNCIKLIFQGPI

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12567
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 47601 Daltons