cDNA Clone Pellino3

• Plasmid: pEBG6P-Pellino3
• Parent Plasmid: pEBG6P
• Expressed: GST-Pellino3
• Genbank: AAH21256.1
• Tag: GST

Sequence of Insert

GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGATGCTGTGAGGAAGGAGGTGAGGAAACCGAGGCTCAGAGAGG
GGAAGTGACTGGCCCAAGGGCACACAGCTGCTACAATGGTTGTCTGGCAA
GTGGGGACAAGGGCCGCCGGCGAAGCCGCCTGGCACTGAGCCGCCGGTCG
CACGCCAACGGGGTGAAGCCAGACGTCATGCACCACATCTCCACGCCGCT
CGTCTCCAAGGCACTGAGTAACCGTGGTCAGCACAGCATCTCGTATACAC
TGTCCCGGAGCCACTCGGTCATAGTGGAGTATACACATGATAGCGACACA
GACATGTTCCAGATTGGCCGCTCCACAGAGAACATGATTGACTTCGTGGT
AACAGACACGTCCCCTGGAGGAGGGGCTGCCGAGGGCCCTTCTGCCCAGA
GCACCATCTCCCGCTATGCCTGCCGCATCCTCTGTGACCGCCGGCCACCC
TATACTGCCCGCATCTATGCCGCTGGCTTCGACGCCTCTAGCAACATCTT
CCTTGGAGAGCGAGCGGCCAAATGGCGGACCCCAGATGGCCTGATGGATG
GACTGACCACCAATGGAGTCCTGGTGATGCACCCGGCAGGCGGCTTCTCC
GAGGACTCAGCCCCGGGTGTCTGGCGGGAGATCTCGGTCTGTGGGAATGT
GTACACATTGCGGGACAGCCGCTCAGCCCAGCAGCGGGGCAAGCTGGTAG
GTGGCCCGCTCCATTCCCCACCCCTATCCTTGCCAGGCCCTCACAAGCTG
CCCATCCCCCTGCCCAAGCCAGGAGATGATCTCCAGTCCTTCTGCCCCAG
GGAGCCCCAACAGGCTCCATATCAGGGAATCCCAGGCCCAGGGGGTTCTT
AGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVLEGNPEVGSPR
TSDLQHRGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGCCEEGGEETEAQ
RGEVTGPRAHSCYNGCLASGDKGRRRSRLALSRRSHANGVKPDVMHHIST
PLVSKALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDF
VVTDTSPGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSN
IFLGERAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCG
NVYTLRDSRSAQQRGKLVGGPLHSPPLSLPGPHKLPIPLPKPGDDLQSFC
PREPQQAPYQGIPGPGGS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12540
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 62755 Daltons