cDNA Clone Pellino3b

• Plasmid: pCMV-HA Pellino3b H396S C398S
• Parent Plasmid: pCMV5 HA1
• Expressed: HA Pellino3b H396S C398S
• Genbank: AAO49466.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGCTACAATGGTTGTCTGGCAAGTGGGGACAAGGGCCGCCGGCG
AAGCCGCCTGGCACTGAGCCGCCGGTCGCACGCCAACGGGGTGAAGCCAG
ACGTCATGCACCACATCTCCACGCCGCTCGTCTCCAAGGCACTGAGTAAC
CGTGGTCAGCACAGCATCTCGTATACACTGTCCCGGAGCCACTCGGTCAT
AGTGGAGTATACACATGATAGCGACACAGACATGTTCCAGATTGGCCGCT
CCACAGAGAACATGATTGACTTCGTGGTAACAGACACGTCCCCTGGAGGA
GGGGCTGCCGAGGGCCCTTCTGCCCAGAGCACCATCTCCCGCTATGCCTG
CCGCATCCTCTGTGACCGCCGGCCACCCTATACTGCCCGCATCTATGCCG
CTGGCTTCGATGCCTCTAGCAACATCTTCCTTGGAGAGCGAGCGGCCAAA
TGGCGGACCCCAGATGGCCTGATGGATGGACTGACCACCAATGGAGTCCT
GGTGATGCACCCGGCAGGCGGCTTCTCCGAGGACTCAGCCCCGGGTGTCT
GGCGGGAGATCTCGGTCTGTGGGAATGTGTACACATTGCGGGACAGCCGC
TCAGCCCAGCAGCGGGGCAAGCTGGTGGAAAACGAGTCCAACGTGCTGCA
GGACGGCTCTCTCATCGACCTGTGTGGGGCCACACTGCTGTGGCGCACAC
CGGCGGGGCTGCTGCGGGCTCCCACACTGAAGCAACTGGAGGCCCAGCGG
CAGGAGGCAAATGCAGCGCGGCCCCAGTGCCCCGTGGGCCTCAGCACTCT
GGCCTTCCCCAGCCCAGCCCGTGGCCGCACAGCGCCCGACAAACAGCAGC
CCTGGGTCTACGTCCGCTGCGGGCACGTCCATGGCTACCACGGCTGGGGC
TGCCGGCGGGAGCGGGGCCCCCAGGAGCGCGAATGTCCTCTCTGCCGCCT
TGTGGGGCCTTATGTGCCTCTATGGCTTGGCCAGGAGGCCGGCCTCTGCC
TGGACCCTGGGCCGCCTAGCCATGCCTTTGCACCTTGCGGCAGCGTCAGC
TCTGAGAAGACTGCCCGCTACTGGGCCCAGACACCACTGCCCCACGGCAC
CCATGCTTTCCATGCCGCCTGCCCCTTTTGCGGGGCCTGGCTTACCGGCG
AGCATGGCTGCGTCCGCCTCATTTTCCAGGGCCCGCTGGATTAGGCGGCC
GC

Amino Acid Sequence

MYPYDVPDYAGSMVLEGNPEVGSPRTSDLQHRGNKGSCVLSSPGEDAQPG
EEPIKYGELIVLGYNGCLASGDKGRRRSRLALSRRSHANGVKPDVMHHIS
TPLVSKALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMID
FVVTDTSPGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASS
NIFLGERAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVC
GNVYTLRDSRSAQQRGKLVENESNVLQDGSLIDLCGATLLWRTPAGLLRA
PTLKQLEAQRQEANAARPQCPVGLSTLAFPSPARGRTAPDKQQPWVYVRC
GHVHGYHGWGCRRERGPQERECPLCRLVGPYVPLWLGQEAGLCLDPGPPS
HAFAPCGSVSSEKTARYWAQTPLPHGTHAFHAACPFCGAWLTGEHGCVRL
IFQGPLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12481
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 49528 Daltons