cDNA Clone Pellino3b

• Plasmid: pEBG6P-Pellino3b H396S C398S
• Parent Plasmid: pEBG6P
• Expressed: GST-Pellino3b H396S C398S
• Genbank: AAO49466.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTC
AGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCG
GTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATC
GTCCTGGGCTACAATGGTTGTCTGGCAAGTGGGGACAAGGGCCGCCGGCG
AAGCCGCCTGGCACTGAGCCGCCGGTCGCACGCCAACGGGGTGAAGCCAG
ACGTCATGCACCACATCTCCACGCCGCTCGTCTCCAAGGCACTGAGTAAC
CGTGGTCAGCACAGCATCTCGTATACACTGTCCCGGAGCCACTCGGTCAT
AGTGGAGTATACACATGATAGCGACACAGACATGTTCCAGATTGGCCGCT
CCACAGAGAACATGATTGACTTCGTGGTAACAGACACGTCCCCTGGAGGA
GGGGCTGCCGAGGGCCCTTCTGCCCAGAGCACCATCTCCCGCTATGCCTG
CCGCATCCTCTGTGACCGCCGGCCACCCTATACTGCCCGCATCTATGCCG
CTGGCTTCGATGCCTCTAGCAACATCTTCCTTGGAGAGCGAGCGGCCAAA
TGGCGGACCCCAGATGGCCTGATGGATGGACTGACCACCAATGGAGTCCT
GGTGATGCACCCGGCAGGCGGCTTCTCCGAGGACTCAGCCCCGGGTGTCT
GGCGGGAGATCTCGGTCTGTGGGAATGTGTACACATTGCGGGACAGCCGC
TCAGCCCAGCAGCGGGGCAAGCTGGTGGAAAACGAGTCCAACGTGCTGCA
GGACGGCTCTCTCATCGACCTGTGTGGGGCCACACTGCTGTGGCGCACAC
CGGCGGGGCTGCTGCGGGCTCCCACACTGAAGCAACTGGAGGCCCAGCGG
CAGGAGGCAAATGCAGCGCGGCCCCAGTGCCCCGTGGGCCTCAGCACTCT
GGCCTTCCCCAGCCCAGCCCGTGGCCGCACAGCGCCCGACAAACAGCAGC
CCTGGGTCTACGTCCGCTGCGGGCACGTCCATGGCTACCACGGCTGGGGC
TGCCGGCGGGAGCGGGGCCCCCAGGAGCGCGAATGTCCTCTCTGCCGCCT
TGTGGGGCCTTATGTGCCTCTATGGCTTGGCCAGGAGGCCGGCCTCTGCC
TGGACCCTGGGCCGCCTAGCCATGCCTTTGCACCTTGCGGCAGCGTCAGC
TCTGAGAAGACTGCCCGCTACTGGGCCCAGACACCACTGCCCCACGGCAC
CCATGCTTTCCATGCCGCCTGCCCCTTTTGCGGGGCCTGGCTTACCGGCG
AGCATGGCTGCGTCCGCCTCATTTTCCAGGGCCCGCTGGATTAGGCGGCC
GC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVLEGNPEVGSPR
TSDLQHRGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGYNGCLASGDKGR
RRSRLALSRRSHANGVKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHS
VIVEYTHDSDTDMFQIGRSTENMIDFVVTDTSPGGGAAEGPSAQSTISRY
ACRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNG
VLVMHPAGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQRGKLVENESNV
LQDGSLIDLCGATLLWRTPAGLLRAPTLKQLEAQRQEANAARPQCPVGLS
TLAFPSPARGRTAPDKQQPWVYVRCGHVHGYHGWGCRRERGPQERECPLC
RLVGPYVPLWLGQEAGLCLDPGPPSHAFAPCGSVSSEKTARYWAQTPLPH
GTHAFHAACPFCGAWLTGEHGCVRLIFQGPLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12475
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 75586 Daltons