cDNA Clone PIP5K2B

• Plasmid: pET156P PI5P4 Kinase II beta 116-416
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His PI5P4 Kinase II beta 116-416
• Genbank: 1BO1_A
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGATTACCAGAATTCAGTGACGCGCAGCGCCCCCATCAACAGTGA
CAGCCAGGGTCGGTGTGGCACGCGTTTCCTCACCACCTACGACCGGCGCT
TTGTCATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATGCACAACATC
TTAAAGAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACACGCTTTT
GCCACAGTTCCTGGGCATGTACCGCCTGACCGTGGATGGTGTGGAAACCT
ACATGGTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACTGTGCATCGC
AAGTATGACCTCAAGGGTTCTACGGTTGCCAGAGAAGCGAGCGACAAGGA
GAAGGCCAAGGACTTGCCAACATTCAAAGACAATGACTTCCTCAATGAAG
GGCAGAAGCTGCATGTGGGAGAGGAGAGTAAAAAGAACTTCCTGGAGAAA
CTGAAGCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGGACTACAG
CCTGCTGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAGGAGATGG
AGGTGGAGGAGCGGGCAGAGGACGAGGAGTGTGAGAATGATGGGGTGGGT
GGCAACCTACTCTGCTCCTATGGCACACCTCCGGACAGCCCTGGCAACCT
CCTCAGCTTTCCTCGGTTCTTTGGTCCTGGGGAATTCGACCCCTCTGTTG
ACGTCTATGCCATGAAAAGCCATGAAAGTTCCCCCAAGAAGGAGGTGTAT
TTTATGGCCATCATTGATATCCTCACGCCATACGATACCAAGAAGAAAGC
TGCACATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAGATCTCGA
CTGTGAACCCTGAGCAGTACTCCAAACGCTTCAACGAGTTTATGTCCAAC
ATCCTGACGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHPSSGLEVLFQGPGSDYQNSVTRSAPINSDSQGRCGTRFLT
TYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTV
DGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDN
DFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMDYSLLVGIHDVDRA
EQEEMEVEERAEDEECENDGVGGNLLCSYGTPPDSPGNLLSFPRFFGPGE
FDPSVDVYAMKSHESSPKKEVYFMAIIDILTPYDTKKKAAHAAKTVKHGA
GAEISTVNPEQYSKRFNEFMSNILT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12472
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; ; Estimated MW of expressed protein : 36755 Daltons