cDNA Clone PIP5K2B

• Plasmid: pET156P PI5P4 Kinase II beta 47-416
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His PI5P4 Kinase II beta 47-416
• Genbank: 1BO1_A
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTGGGGGGTGAACCACACGATCAATGAGCTGAGCAATGTTCC
TGTTCCTGTCATGCTAATGCCAGATGACTTCAAAGCCTACAGCAAGATCA
AGGTGGACAATCATCTCTTCAATAAGGAGAACCTGCCCAGCCGCTTTAAG
TTCAAGGAGTATTGCCCCATGGTGTTCCGAAACCTTCGGGAGAGGTTTGG
AATTGATGATCAGGATTACCAGAATTCAGTGACGCGCAGCGCCCCCATCA
ACAGTGACAGCCAGGGTCGGTGTGGCACGCGTTTCCTCACCACCTACGAC
CGGCGCTTTGTCATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATGCA
CAACATCTTAAAGAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACA
CGCTTTTGCCACAGTTCCTGGGCATGTACCGCCTGACCGTGGATGGTGTG
GAAACCTACATGGTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACTGT
GCATCGCAAGTATGACCTCAAGGGTTCTACGGTTGCCAGAGAAGCGAGCG
ACAAGGAGAAGGCCAAGGACTTGCCAACATTCAAAGACAATGACTTCCTC
AATGAAGGGCAGAAGCTGCATGTGGGAGAGGAGAGTAAAAAGAACTTCCT
GGAGAAACTGAAGCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGG
ACTACAGCCTGCTGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAG
GAGATGGAGGTGGAGGAGCGGGCAGAGGACGAGGAGTGTGAGAATGATGG
GGTGGGTGGCAACCTACTCTGCTCCTATGGCACACCTCCGGACAGCCCTG
GCAACCTCCTCAGCTTTCCTCGGTTCTTTGGTCCTGGGGAATTCGACCCC
TCTGTTGACGTCTATGCCATGAAAAGCCATGAAAGTTCCCCCAAGAAGGA
GGTGTATTTCATGGCCATCATTGATATCCTCACGCCATACGATACTAAGA
AGAAAGCTGCACATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAG
ATCTCGACTGTGAACCCTGAGCAGTACTCCAAACGCTTCAACGAGTTTAT
GTCCAACATCCTGACGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMWGVNHTINELSNVPVPVMLMPDDFKA
YSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTR
SAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVE
CHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVA
REASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQ
LKIMDYSLLVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGTP
PDSPGNLLSFPRFFGPGEFDPSVDVYAMKSHESSPKKEVYFMAIIDILTP
YDTKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFNEFMSNILT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12471
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; ; Estimated MW of expressed protein : 44901 Daltons