cDNA Clone TRIM25

• Plasmid: pEBG6P-TRIM 25
• Parent Plasmid: pEBG6P
• Expressed: GST-TRIM 25
• Genbank: NP_005073.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTC
CATCTGCCTGGAGCCCTTCAAGGAGCCGGTCACCACTCCGTGCGGCCACA
ACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCCA
TACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCA
CAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACC
TGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCT
GCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGC
CGCCGTGAAGACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACC
TGCAGCCGCACTTCGACAGCCCCGCCTTCCAGGACCACCCGCTGCAGCCG
CCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGCG
GGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGG
TGGAGCATAAGACCTGCTCTCCCGCGTCCCTGAGCCAGGCCAGCGCCGAC
CTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTACAGTCAGATCAA
CGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGC
GGATGACTGCAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAA
ATGAAGGCTCTCTTGGACGCCTCAGAGACCACCTCGACAAGGAAGATAAA
GGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATTC
TCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAG
AGCCTGACCAAGAGGGATGAGTTCGAGTTTCTGGAGAAAGCATCAAAACT
GCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGTGGAACTGAACC
ACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAG
CTGAAGCAGTGCATCGGGCGGCTCCAGGAGCTCACCCCCAGTTCAGGTGA
CCCTGGAGAGCATGACCCAGCGTCCACACACAAATCCACACGCCCTGTGA
AGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCCT
GCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGA
TTTAAAACAAGCTGGCTTGGAGGCTGCAGCCAAAGCCACCAGCTCACATC
CGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCTTCCTGGCCAAG
TCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAA
CACCGCCCACAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTG
TGGCTGAGATGCCTCAGAACTACCGGCCGCATCCCCAGAGGTTCACATAC
TGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACTG
GGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACG
GAAGCATGAACCGGCAGGGCCCAGAAAGCAGGCTCGGCCGCAACAGCGCC
TCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCCTGGCACAATAA
CGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTC
TCAACTGTGACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTC
CACCTGATGTATAAGTTCAGGGTGGACTTTACTGAGGCTTTGTACCCGGC
TTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAGT
AGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAEL
CPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCR
AVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQ
VACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLR
RKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRH
KLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDA
SETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDE
FEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGR
LQELTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLP
TFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLE
YYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGL
HCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWF
NTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFR
VDFTEALYPAFWVFSAGATLSICSPK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12453
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 99191 Daltons