cDNA Clone TRIM25

• Synonyms: *
• Plasmid: pCMV-HA-2 TRIM 25
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-TRIM 25
• Genbank: NP_005073.2
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTC
CATCTGCCTGGAGCCCTTCAAGGAGCCGGTCACCACTCCGTGCGGCCACA
ACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCCA
TACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCA
CAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACC
TGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCT
GCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGC
CGCCGTGAAGACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACC
TGCAGCCGCACTTCGACAGCCCCGCCTTCCAGGACCACCCGCTGCAGCCG
CCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGCG
GGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGG
TGGAGCATAAGACCTGCTCTCCCGCGTCCCTGAGCCAGGCCAGCGCCGAC
CTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTACAGTCAGATCAA
CGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGC
GGATGACTGCAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAA
ATGAAGGCTCTCTTGGACGCCTCAGAGACCACCTCGACAAGGAAGATAAA
GGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATTC
TCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAG
AGCCTGACCAAGAGGGATGAGTTCGAGTTTCTGGAGAAAGCATCAAAACT
GCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGTGGAACTGAACC
ACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAG
CTGAAGCAGTGCATCGGGCGGCTCCAGGAGCTCACCCCCAGTTCAGGTGA
CCCTGGAGAGCATGACCCAGCGTCCACACACAAATCCACACGCCCTGTGA
AGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCCT
GCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGA
TTTAAAACAAGCTGGCTTGGAGGCTGCAGCCAAAGCCACCAGCTCACATC
CGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCTTCCTGGCCAAG
TCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAA
CACCGCCCACAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTG
TGGCTGAGATGCCTCAGAACTACCGGCCGCATCCCCAGAGGTTCACATAC
TGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACTG
GGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACG
GAAGCATGAACCGGCAGGGCCCAGAAAGCAGGCTCGGCCGCAACAGCGCC
TCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCCTGGCACAATAA
CGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTC
TCAACTGTGACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTC
CACCTGATGTATAAGTTCAGGGTGGACTTTACTGAGGCTTTGTACCCGGC
TTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAGT
AGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMAELCPLAEELSCSICLEPFKEPVTTP
CGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFL
QADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFC
QEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICH
ICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQ
QDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTI
YQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEV
ELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKST
RPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKAT
SSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYT
VASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVG
ICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRV
GVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSIC
SPK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV-HA-2 TRIM 25907.69 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12451
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 73351 Daltons
• Low Temp: No