cDNA Clone PIP5K2B

• Plasmid: pET28 PI5P4 Kinase II beta
• Parent Plasmid: pET28b original
• Genbank: 1BO1_A
• Species: Human

Sequence of Insert

GGATCCATGTCGTCCAACTGCACCAGCACCACGGCGGTGGCGGTGGCGCC
GCTCAGCGCCAGCAAGACCAAGACCAAGAAGAAGCATTTCGTGTGCCAGA
AAGTGAAGCTATTCCGGGCCAGCGAGCCGATCCTCAGCGTCCTGATGTGG
GGGGTGAACCACACGATCAATGAGCTGAGCAATGTTCCTGTTCCTGTCAT
GCTAATGCCAGATGACTTCAAAGCCTACAGCAAGATCAAGGTGGACAATC
ATCTCTTCAATAAGGAGAACCTGCCCAGCCGCTTTAAGTTTAAGGAGTAT
TGCCCCATGGTGTTCCGAAACCTTCGGGAGAGGTTTGGAATTGATGATCA
GGATTACCAGAATTCAGTGACGCGCAGCGCCCCCATCAACAGTGACAGCC
AGGGTCGGTGTGGCACGCGTTTCCTCACCACCTACGACCGGCGCTTTGTC
ATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATGCACAACATCTTAAA
GAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACACGCTTTTGCCAC
AGTTCCTGGGCATGTACCGCCTGACCGTGGATGGTGTGGAAACCTACATG
GTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACTGTGCATCGCAAGTA
TGACCTCAAGGGTTCTACGGTTGCCAGAGAAGCGAGCGACAAGGAGAAGG
CCAAGGACTTGCCAACATTCAAAGACAATGACTTCCTCAATGAAGGGCAG
AAGCTGCATGTGGGAGAGGAGAGTAAAAAGAACTTCCTGGAGAAACTGAA
GCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGGACTACAGCCTGC
TGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAGGAGATGGAGGTG
GAGGAGCGGGCAGAGGACGAGGAGTGTGAGAATGATGGGGTGGGTGGCAA
CCTACTCTGCTCCTATGGCACACCTCCGGACAGCCCTGGCAACCTCCTCA
GCTTTCCTCGGTTCTTTGGTCCTGGGGAATTCGACCCCTCTGTTGACGTC
TATGCCATGAAAAGCCATGAAAGTTCCCCCAAGAAGGAGGTGTATTTTAT
GGCCATCATTGATATCCTCACGCCATACGATACCAAGAAGAAAGCTGCAC
ATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAGATCTCGACTGTG
AACCCTGAGCAGTACTCCAAACGCTTCAACGAGTTTATGTCCAACATCCT
GACGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSMSSNCTSTTAVAVAPLSASKTKTKKKHFVCQ
KVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDN
HLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDS
QGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLP
QFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEK
AKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMDYSL
LVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGTPPDSPGNLL
SFPRFFGPGEFDPSVDVYAMKSHESSPKKEVYFMAIIDILTPYDTKKKAA
HAAKTVKHGAGAEISTVNPEQYSKRFNEFMSNILT*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU12421
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 49402 Daltons