cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pEBG6P-TPL2 S62A T290A
• Parent Plasmid: pEBG6P
• Expressed: GST-TPL2 S62A T290A
• Genbank: P41279
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTT
ATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAATC
TTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATG
TGTCAAGACAGTAATCAAAACGATGAGCGTTCTAAGGCTCTGCTGCTTAG
TGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATATGGAACTGTGGAGG
ATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTAT
GGACAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCC
CCAAAATGGACGTTACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGA
AGCTGACTTACAGGAATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTT
GGAAAGGTATACTTGGCACAAGATATAAAGACGAAGAAAAGAATGGCGTG
TAAACTGATCCCTGTAGATCAATTTAAGCCATCTGATGTGGAAATCCAGG
CTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGG
GGTGAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCT
GGAGAAACTGGAGAGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGG
TGACAAAGCATGTTCTCAAGGGACTTGATTTTCTACACTCAAAGAAAGTG
ATCCATCATGATATTAAACCTAGCAACATTGTTTTCATGTCCACAAAAGC
TGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGATGTCTATT
TTCCTAAGGACCTCCGAGGAGCAGAGATTTACATGAGCCCAGAGGTCATC
CTGTGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCAC
GCTCATCCACATGCAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTC
GCTCAGCCTATCCCTCCTACCTGTACATAATCCACAAGCAAGCACCTCCA
CTGGAAGACATTGCAGATGACTGCAGTCCAGGGATGAGAGAGCTGATAGA
AGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCAGACCTAC
TAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTACG
AGTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGA
GCTGGAACTTCCTGAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCG
AGGAATCTGAGATGCTCAAGAGGCAACGCTCTCTCTACATCGACCTCGGC
GCTCTGGCCGGCTACTTCAATCTTGTTCGGGGACCACCAACGCTTGAATA
TGGCTGAGAATTCAAAGGCCTACGTCGACGAGCTCACTAGTCGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSEYMSTGSDNKEEI
DLLIKHLNVSDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKALL
LSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVI
TPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRM
ACKLIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGS
VLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMST
KAVLVDFGLSVQMTEDVYFPKDLRGAEIYMSPEVILCRGHSTKADIYSLG
ATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMREL
IEASLERNPNHRPRAADLLKHEALNPPREDQPRCTSLDSALLERKRLLSR
KELELPENIADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTL
EYG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12363
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 80136 Daltons