cDNA Clone VPS15

• Plasmid: pGEX6P-2-VPS15
• Parent Plasmid: pGEX6P2
• Expressed: GST-VPS15
• Genbank: NP_055417
• Tag: GST

Sequence of Insert

GCGGCCGCGATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCT
TTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAA
GCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAA
GGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTT
AACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCTG
CACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAA
GCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCAT
CAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCC
AGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGG
GACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTCT
AACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGG
CAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATT
GCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATA
TATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAA
CAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTG
ATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCAACT
TTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAAA
TTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAG
CCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAA
TGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGT
TTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGG
AAGGATTTGGGCAACATTATTCACAATCTCTGTGGACATGATCTGCCAGA
AAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTATCTG
TTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGCT
GCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCT
TTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTGTTC
CTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCTC
GTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTCT
GCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTAG
CCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCTG
GAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATGA
AGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCC
AAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGAC
CCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACGGCT
GTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCACA
TGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTT
TTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTC
AATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTTG
TCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTG
CTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTTCCT
GTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCACAG
TGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATGCCT
TATCTTGACCCATATATTACCCAACCAATAATACAGATTGAAAGAAAACT
TGTTCTGCTCAGTGTTTTAAAGGAACCAGTAAGTCGTTCTATATTTGATT
ATGCTTTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCACATG
CGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCTCCGCCAGAGGA
TCCTGCCATAGCACAGCTTCTGAAGAAGTTGCTCTCACAGGGAATGACAG
AGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCATGATGAAATCT
AATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAGTAG
TCAGAAAGGTGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGACAAG
TTGATCTTGTTAAAACCAAACAAGAACCAGATGACAAACGGGCCAGAAAA
CATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAAAGCATGTTTGG
GTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTGATC
AGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCCGAGTCCTCTGCTGGC
ATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACAACTGT
CCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTACCAT
CCACCTATCAGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAACTC
ATCCAGCAAAAGCGGGAGCAGTGCAATGCTGAGAGAATAGCTAAGCAGAT
GATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGCGTC
CTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTGAAT
CGAATTAGAGTCTCTGATGAACACTCACTTTTTGCAACATGTTCAAATGA
TGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGGGAAGACCACCA
CTACCAGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAGACG
CTCACATTCTGCCAAGGCTCCCACTATTTAGCCATAGCATCTGATAATGG
TGCTGTCCAGCTTCTTGGAATTGAGGCTTCTAAGCTGCCCAAGTCTCCTA
AAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGTTGT
GTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGCCTA
TGCCACTGTGAATGGCTCTCTGGTTGGCTGGGACCTTAGGTCTTCAAGCA
ATGCGTGGACTTTAAAGCATGATTTAAAGTCGGGCCTCATCACTTCCTTT
GCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACAAGCAGTGGTAC
CATGGCTTGTTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACTGTC
ATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATGCACCCTCTGTATCAG
TCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTGGGA
CATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCACCAC
CACTTTCTGAATTACAGCCTTCTCCTCATAGCGTCCATGGTATCTACTGT
AGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGCTCAGATATGAA
AATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTGCAG
GAAGTACTAGTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAAGGC
ACTGAAGTTGTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCAAGTGA
TGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATGACA
TCATCACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTAACT
GCTTCTAGAGATGGGATTGTGAAGGTGTGGAAATAAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAMGNQLAGIA
PSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQD
PTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDN
LYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSW
NWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFA
TELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLF
DLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLK
QQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLCG
HDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRL
SVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIY
PEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNMEN
DPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMEN
GITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVG
WQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASD
IAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQPIIQ
IERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDC
PPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVDQSH
LHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVKQDSNVNEEW
KSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVP
EVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAER
IAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFA
TCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHYLAI
ASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQ
SVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIG
TSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAVQGNNE
VSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTA
GSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQK
VGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12349
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 180829 Daltons