cDNA Clone PLK

• Plasmid: pFBHTb tb PLK
• Parent Plasmid: pFastbac HTc
• Genbank: XP_846254.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCACGCAACCGCTGAGACGTGTGAAACGCCGTCGAGTTCGCGGCGC
CCTCCAAATGACAGGCCGGACTTTCGTGAGGGTTCAACTCTTAAGGAGTT
TGATAAAAGTGGCCGTCTCGTGGGATATTTCCGTTGCGGAAGGATGCTCG
GGCGTGGTGGCTTTGCAAAGTGTTATGAAGTGGAGCAGGGTGGCGATACG
TATGCGTTGAAGGTTGTGGACCGCTCACTACTGCAGAAAACTAAGACACT
ACAGAAACTCCACTCTGAGATATCCATTCACCGCCGCATGAAGCACAAAC
ACATTGTTAACTTCATACGGACCTTCCATGACGACTGGAATGTTTATATT
CTGTTGGAGAAGTGCAGCAATCAGACGCTGATGGAAATATTGAAGCGCCG
GCAACGTTTCAGTGTGCCAGAGACACAATACATTGCATTGCAGTCGCTTT
CCGCCATTCAATATATGCATGAGCAATGTGTTATTCATCGTGACCTGAAA
CTTGGTAACATAATGATGGATGCAAATATGAACGTGAAGATTGGGGACTT
CGGTTTGGCTGCTGAGTTGCAGTACGACGGGGAGCGGAAACGCACTATTT
GTGGCACGCCCAATTATATCGCACCGGAGATCATTGAAGGTTCGCGCGAG
GGACATAGTTACGAAGTGGATGTGTGGTCGCTCGGTGTGATCCTCTACAC
GCTGTTGGTTGGAGAGCCGCCGTTTCAGACATCAGATGTGAAGGCCACTT
ACCGTCGTATTAGGCAATGCCGGTATGAATTTCCTTCACATGTAGATGTG
CCAGAGAGTGGGAAGGAACTTATTCACAGTATTCTGCAGAGCCGCCCCGA
CCAACGGCCGACGTTGCTGGAAATTCGTTCACATCCATTTTTCCGCCTCC
CGCCGCCACCGACGACAGCACCTACCACACTCTTCTACAGTTCACGCCGC
CGTCAGCATTCGGATGACCCCCGAGGACATGCGCAGGGACCACTTCCACT
TCGCCGCCAAAAATCAGGGGACATTCAGGCGGCGTTGCAGAAACAAACAC
CGCAACGCCGTCAACCGCAGTCGCAGCCCAAATCAGTTGAAGCTGTTCGC
TGCATTTCGAGTCCGCGTGTGTCCCGCGAAGTGCTTCAACCTATCAGCAC
AAATCTACCCAAAACGGACCGATACCACCTAAAACCCAGCTGCCCGGCGG
TAGCTTCCGCAAGGTTTCATGGGGCTTTGGGCGGTGACTGCAACAACAAC
AACAACAACAATAACAATAACATCAACAACAACAACAACAACAACATTAA
CAGTGCTGCGGTGAGCATCCCGTCACCTCGTGCAGATGCGATTCGACCCC
TCACGCAAGTTGCCGCACCGGGCGGTGCTGATGAATCCGCGGAAACCACT
GCAACAACTCCGCGTGTGCAGCTGAGACCTCACCCAGCTATAGAGGAAGA
GGAGAAGAATGAACTCACCGCCGTGCATGATCAACTTCATCAGACGTTAC
GGGAAATAGGCGACATTTCCCCACGTGAGGTTGCCACCCGCACACAAACC
AAACGGTCACCGCAAGTGGCTCCCTTGCCAGTGGCGAACTCACCCACAAC
ATCTACAGTTCCTGCATTAACGCCCAGATGCGAAGACGAGCCGGCGCCAT
CGTTGCCACTTCCCACTGTTTGGGTGACGAGTTTCGCGGATTTCTCCGAA
AAATACGGGTTGTGTTACCGGTTGAGCACCGGCCATACCGGTGTGCACTT
CAACGACTCCACTAAAATGGTGTGGGAGCCCATTACAAACCGTGTAGAGT
ATTATATGCGAGTGAAGGAAGTTGTTGCGCGTGGTAATGCCAACGTCCTG
CAGGCGCGAGATCAACAACATGCGTTTCACATGGATATGTTCCCTGAAAG
CCTGACCAAGAAGGTGACATTGATCAAATACTTTAAATCATACCTCTCAC
GAACGCGTAATTCTCATACCAACGTGGAGGTTGTACGGTGCTCACCATAT
GTGTCCGAAGTTACTCCGTCACTTAGCGGACCGCACATGATTGAAGATAT
TGTATATGTCAAGCGCTGGTTGATCACACCACAAGCAATTATATTCCGTC
TATCCAACAAAACTATTCAGGTTTGTTTTCATGATAAGGCGGAGGTTATT
CTAAGTTCGGAGTCGCGTGTTGTTACATATACAGAACCAACTGGCAACCG
CGTGACAATGTCGCTATCGTCTGTTGCGACACGCTCTAGAGAAATTGCTG
CACGGCTTCGTTACACCAAAGACATCCTCAGCGAGCTCATACAAAACCGT
GATATTTAGCTCGAG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIHATAETCETPSSSRRPPNDRPD
FREGSTLKEFDKSGRLVGYFRCGRMLGRGGFAKCYEVEQGGDTYALKVVD
RSLLQKTKTLQKLHSEISIHRRMKHKHIVNFIRTFHDDWNVYILLEKCSN
QTLMEILKRRQRFSVPETQYIALQSLSAIQYMHEQCVIHRDLKLGNIMMD
ANMNVKIGDFGLAAELQYDGERKRTICGTPNYIAPEIIEGSREGHSYEVD
VWSLGVILYTLLVGEPPFQTSDVKATYRRIRQCRYEFPSHVDVPESGKEL
IHSILQSRPDQRPTLLEIRSHPFFRLPPPPTTAPTTLFYSSRRRQHSDDP
RGHAQGPLPLRRQKSGDIQAALQKQTPQRRQPQSQPKSVEAVRCISSPRV
SREVLQPISTNLPKTDRYHLKPSCPAVASARFHGALGGDCNNNNNNNNNN
INNNNNNNINSAAVSIPSPRADAIRPLTQVAAPGGADESAETTATTPRVQ
LRPHPAIEEEEKNELTAVHDQLHQTLREIGDISPREVATRTQTKRSPQVA
PLPVANSPTTSTVPALTPRCEDEPAPSLPLPTVWVTSFADFSEKYGLCYR
LSTGHTGVHFNDSTKMVWEPITNRVEYYMRVKEVVARGNANVLQARDQQH
AFHMDMFPESLTKKVTLIKYFKSYLSRTRNSHTNVEVVRCSPYVSEVTPS
LSGPHMIEDIVYVKRWLITPQAIIFRLSNKTIQVCFHDKAEVILSSESRV
VTYTEPTGNRVTMSLSSVATRSREIAARLRYTKDILSELIQNRDI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12335
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 90234 Daltons