cDNA Clone PLK

• Plasmid: pFBHTc PLK 2-294 T Brucei
• Parent Plasmid: pFastbac HTc
• Genbank: XP_846254.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCACGCAACCGCTGAGACGTGTGAAACGCCGTCGAGTTCGCGGCGC
CCTCCAAATGACAGGCCGGACTTTCGTGAGGGTTCAACTCTTAAGGAGTT
TGATAAAAGTGGCCGTCTCGTGGGATATTTCCGTTGCGGAAGGATGCTCG
GGCGTGGTGGCTTTGCAAAGTGTTATGAAGTGGAGCAGGGTGGCGATACG
TATGCGTTGAAGGTTGTGGACCGCTCACTACTGCAGAAAACTAAGACACT
ACAGAAACTCCACTCTGAGATATCCATTCACCGCCGCATGAAGCACAAAC
ACATTGTTAACTTCATACGGACCTTCCATGACGACTGGAATGTTTATATT
CTGTTGGAGAAGTGCAGCAATCAGACGCTGATGGAAATATTGAAGCGCCG
GCAACGTTTCAGTGTGCCAGAGACACAATACATTGCATTGCAGTCGCTTT
CCGCCATTCAATATATGCATGAGCAATGTGTTATTCATCGTGACCTGAAA
CTTGGTAACATAATGATGGATGCAAATATGAACGTGAAGATTGGGGACTT
CGGTTTGGCTGCTGAGTTGCAGTACGACGGGGAGCGGAAACGCACTATTT
GTGGCACGCCCAATTATATCGCACCGGAGATCATTGAAGGTTCGCGCGAG
GGACATAGTTACGAAGTGGATGTGTGGTCGCTCGGTGTGATCCTCTACAC
GCTGTTGGTTGGAGAGCCGCCGTTTCAGACATCAGATGTGAAGGCCACTT
ACCGTCGTATTAGGCAATGCCGGTATGAATTTCCTTCACATGTAGATGTG
CCAGAGAGTGGGAAGGAACTTATTCACAGTATTCTGCAGAGCCGCCCCGA
CCAACGGCCGACGTTGCTGGAAATTCGTTCACATTAGCTCGAG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIHATAETCETPSSSRRPPNDRPD
FREGSTLKEFDKSGRLVGYFRCGRMLGRGGFAKCYEVEQGGDTYALKVVD
RSLLQKTKTLQKLHSEISIHRRMKHKHIVNFIRTFHDDWNVYILLEKCSN
QTLMEILKRRQRFSVPETQYIALQSLSAIQYMHEQCVIHRDLKLGNIMMD
ANMNVKIGDFGLAAELQYDGERKRTICGTPNYIAPEIIEGSREGHSYEVD
VWSLGVILYTLLVGEPPFQTSDVKATYRRIRQCRYEFPSHVDVPESGKEL
IHSILQSRPDQRPTLLEIRSH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 37254 Daltons