cDNA Clone SAP97 (rat)

• Plasmid: pCMV-HA SAP97 S122A S158A T176A S410A S415A
• Parent Plasmid: pCMV5
• Expressed: HA SAP97 S122A S158A T176A S410A S415A
• Genbank: AM710296.2 with residue change S23N
• Tag: HA
• Species: Rat

Sequence of Insert

GAATTCGCCCTTGCGGGTACCCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCATCTGTTGGAAGAATATCGGTCGAAACTAAACCAAACCGAAGACAGACAGCTCAGGAGTTCCATAGAGCGGGTTATTAGCATATTTCAGAGCAACCTCTTTCAGGCTTTAATAGACATTCAAGAATTTTATGAAGTGACCTTACTTGATAATCCAAAATGTGTGGATCATTCAAAGCAGTGTGAACCGGTCCAACCCGGGAATCCTTGGGAGAGTGGCAGCCTTTCAAGTGCTGCTGTGACTTCAGAGAGCCTGCCCGGCGGCCTTAGCCCTCCAGTAGAGAAATACCGGTATCAGGACGAAGAGGTGCTGCCTTCAGAGCGGATTGCCCCGCAAGTCCCAAATGAGGTGCTGGGTCCGGAGCTGGTTCACGTCTCGGAGAAGAGCCTGTCAGAGATTGAGAATGTCCACGGGTTTGTCTCTCACTCTCATATCGCACCCATAAAGGCAAATCCTCCTCCTGTGCTGGTCAACACAGACAGCTTGGAGGCACCAACTTATGTTAATGGTACTGATGCAGATTATGAATATGAGGAAATCACACTTGAAAGGGGAAATTCAGGCCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACATTGGAGATGACTCAAGTATTTTCATCACCAAAATTATCACGGGAGGAGCAGCTGCCCAGGATGGAAGATTGCGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGATGTTCGTGATGTAACCCACAGCAAAGCAGTTGAAGCATTGAAAGAAGCTGGATCGATAGTGCGATTATATGTGAAAAGGCGGAAGCCAGCCTCAGAAAAAATCATGGAAATAAAACTTATTAAAGGCCCTAAAGGTCTTGGGTTCAGCATTGCTGGAGGTGTTGGAAACCAGCACATTCCTGGGGATAACAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACACAAGGACGGCAAACTTCAGATTGGAGACAAGCTTCTAGCAGTGAACAGTGTCTGTTTAGAAGAAGTTACTCATGAAGAAGCAGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCAACAAGTATGTATATAAATGATGGCTATGCACCACCCGACATCACTAATTCTTCTTCTCAGTCTGTTGACAACCATGTTGCCCCGTCTTCATACTTGGGCCAGACTCCAGCGGCACCAGCCAGATACGCACCCATTTCTAAAGCAGTGCTTGGAGATGATGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACGGGACTTGGTTTCAACATTGTGGGAGGCGAAGATGGAGAAGGGATTTTTATCTCTTTCATCCTTGCTGGGGGACCTGCTGACCTAAGCGGAGAGCTCAGAAAAGGAGATCGCATTATATCGGTAAACAGTGTTGACCTCAGAGCTGCAAGTCATGAACAAGCAGCTGCCGCATTAAAAAACGCAGGCCAAGCTGTCACTATTGTCGCACAGTATCGGCCTGAAGAGTACAGTCGTTTTGAAGCTAAAATTCACGACTTGCGGGAGCAGATGATGAACAGCAGTGTCAGCTCAGGGTCAGGGTCTCTCCGAACCAGCCAAAAGCGGTCCCTCTATGTCAGAGCCCTTTTTGATTACGACAAGACTAAAGACAGTGGCCTTCCCAGTCAAGGGTTGAACTTCAAATTCGGAGATATTCTCCATGTTATCAATGCTTCTGATGACGAGTGGTGGCAGGCCAGACAGGTGACCCCAGATGGTGAGAGTGACGAAGTTGGAGTAATTCCTAGTAAACGCAGAGTTGAGAAGAAAGAACGAGCCCGATTAAAAACAGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTTCCCTTCTACAAGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCTGACCAGCACGTAACTTCTAATGCCAGCGATAGCGAAAGTAGTTACCGTGGTCAAGAAGAATATGTTTTATCGTATGAGCCGGTGAATCAGCAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAACTAGACCAAAGCGTGACTATGAGGTGGATGGACGAGATTACCATTTTGTGACTTCAAGAGAACAGATGGAGAAAGATATCCAGGAGCATAAATTCATTGAAGCTGGCCAGTATAACAACCATCTATACGGAACGAGTGTCCAATCTGTGCGAGCAGTAGCAGAGAAGGGCAAGCATTGTATCCTTGATGTGTCTGGAAATGCCATCAAGAGGTTGCAGATTGCACAGCTCTATCCAATATCTACTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATAAACGCCTAACAGACGAACAGGCCAGAAAAACGTTTGAGAGAGCAGTGAGACTGGAGCAGGAGTTCACTGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACATTTACAATCAAGTGAAGCAGATCATAGAAGAGCAGTCTGGTCCTTACATCTGGGTCCCAGCAAAAGAAAAATTATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIRPCGTPVRKQDTQRALHLLEEYRSKLNQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNP
WESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERIAPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHIAPIKANPPPVLVNTDSLEAPTYVN
GTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKPAS
EKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKVAKPTSMYINDGY
APPDITNSSSQSVDNHVAPSSYLGQTPAAPARYAPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIIS
VNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILH
VINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGSFNDKRKKNLFSRKFPFYKNKDQSEQETSDADQHVTSNASDSESS
YRGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTS
VQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISTFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQS
GPYIWVPAKEKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12326
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression.
• Low Temp: No