cDNA Clone PLK

• Plasmid: pGEX-5X-tb PLK 2-294
• Parent Plasmid: pGEX5X
• Genbank: XP_846254.1
• Species: Trypanosoma brucei TREU927

Sequence of Insert

GGATCCACGCAACCGCTGAGACGTGTGAAACGCCGTCGAGTTCGCGGCGC
CCTCCAAATGACAGGCCGGACTTTCGTGAGGGTTCAACTCTTAAGGAGTT
TGATAAAAGTGGCCGTCTCGTGGGATATTTCCGTTGCGGAAGGATGCTCG
GGCGTGGTGGCTTTGCAAAGTGTTATGAAGTGGAGCAGGGTGGCGATACG
TATGCGTTGAAGGTTGTGGACCGCTCACTACTGCAGAAAACTAAGACACT
ACAGAAACTCCACTCTGAGATATCCATTCACCGCCGCATGAAGCACAAAC
ACATTGTTAACTTCATACGGACCTTCCATGACGACTGGAATGTTTATATT
CTGTTGGAGAAGTGCAGCAATCAGACGCTGATGGAAATATTGAAGCGCCG
GCAACGTTTCAGTGTGCCAGAGACACAATACATTGCATTGCAGTCGCTTT
CCGCCATTCAATATATGCATGAGCAATGTGTTATTCATCGTGACCTGAAA
CTTGGTAACATAATGATGGATGCAAATATGAACGTGAAGATTGGGGACTT
CGGTTTGGCTGCTGAGTTGCAGTACGACGGGGAGCGGAAACGCACTATTT
GTGGCACGCCCAATTATATCGCACCGGAGATCATTGAAGGTTCGCGCGAG
GGACATAGTTACGAAGTGGATGTGTGGTCGCTCGGTGTGATCCTCTACAC
GCTGTTGGTTGGAGAGCCGCCGTTTCAGACATCAGATGTGAAGGCCACTT
ACCGTCGTATTAGGCAATGCCGGTATGAATTTCCTTCACATGTAGATGTG
CCAGAGAGTGGGAAGGAACTTATTCACAGTATTCTGCAGAGCCGCCCCGA
CCAACGGCCGACGTTGCTGGAAATTCGTTCACATTAGCTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLIEGRGIHATAETCETPSSSRRPPNDRPDF
REGSTLKEFDKSGRLVGYFRCGRMLGRGGFAKCYEVEQGGDTYALKVVDR
SLLQKTKTLQKLHSEISIHRRMKHKHIVNFIRTFHDDWNVYILLEKCSNQ
TLMEILKRRQRFSVPETQYIALQSLSAIQYMHEQCVIHRDLKLGNIMMDA
NMNVKIGDFGLAAELQYDGERKRTICGTPNYIAPEIIEGSREGHSYEVDV
WSLGVILYTLLVGEPPFQTSDVKATYRRIRQCRYEFPSHVDVPESGKELI
HSILQSRPDQRPTLLEIRSH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12284
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 60278 Daltons