cDNA Clone PPM1E

• Plasmid: pFBHTb PPM1E 230-755
• Parent Plasmid: pFastBac HTb
• Expressed: 6His PPM1E 230-755
• Genbank: NM_014906.3
• Tag: 6His

Sequence of Insert

GGATCCTATGAGACATCAATCCATGCCATCAAAAACATGCGCAGGAAAAT
GGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCTCTTCAACCTAG
AGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGA
GTAGATGCTGCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCG
CCAGGAGATGTTCCCCCATGATCCTGCTGAGGCCCTGTGCAGGGCCTTCC
GGGTCACTGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGAGAGCTTAAGA
TGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGT
GGCCTGGGTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGCCAAGCTG
TTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGA
ATTGAGGCCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAA
TGGAAGTCTGTCGGTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCAT
ATATCTGTGGGGATGCAGATTCTGCCTCCACTGTTCTGGATGGGACCGAA
GACTACCTCATTCTGGCCTGTGATGGCTTCTATGACACCGTGAACCCTGA
TGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGACA
GCAGCATGGTTGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCA
AGTGATAACATCACGGTTATTGTGGTATTCCTGAGGGACATGAACAAAGC
TGTAAATGTTAGTGAGGAATCAGATTGGACAGAGAACTCTTTTCAAGGAG
GGCAAGAAGATGGTGGGGATGATAAGGAGAATCATGGAGAGTGCAAACGC
CCTTGGCCTCAGCACCAGTGCTCAGCACCAGCCGACCTAGGCTATGATGG
GCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAAA
TCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCA
AGCAAACCTCACAGTGCCCAGTTTTTGCTACCAGTTGAGATGTTTGGTCC
TGGTGCACCAAAGAAAGCAAATCTTATTAATGAGTTAATGATGGAAAAAA
AATCAGTTCAGTCATCATTGCCTGAATGGAGTGGTGCTGGAGAGTTTCCC
ACTGCTTTCAATTTGGGTTCAACAGGGGAGCAGATATACAGAATGCAGAG
CTTGTCTCCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAATCCCCGG
GAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAAG
TGGCACAGATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCA
AGAGCCTTCCCACAAAATAGGCACTAGCCTGTCCTCACTTACTGGAAGTG
GGAAGAGAAATAGGATAAGAAGTTCTCTGCCATGGAGGCAAAATAGTTGG
AAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACACATGATATTCC
ATGCCCAGATCTTCCTTGGAGCTATAAAATAGAATAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSYETSIHAIKNMRRKMEDKHVCI
PDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPHD
PAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQ
VMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSR
AIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVS
DHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEES
DWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTD
RTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKAN
LINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSG
LENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIG
TSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWS
YKIE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12259
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 62340 Daltons