cDNA Clone PPM1E

• Plasmid: pEBG6P-PPM1E
• Parent Plasmid: pEBG6P
• Expressed: GST-PPM1E
• Genbank: CAH18109.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAGTCCTTCAGAGTGATCTTTCTGCACATTATATCCCAAAG
GAAACGGATGGCACAGAAGGGACTGTGGGACCAGGAAGAACAAGCTTACT
TTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCTGCTATTTATGCCTCC
ATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCATGATCC
TGCTGAGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGC
AGAAAGCAGCCAGGGAGAGCTTAAGATGTGGGACCACAGGAGTGGTGACT
TTCATCAGAGGCAACATGCTACATGTGGCCTGGGTGGGTGATTCCCAGGT
TATGCTTGTGAGAAAGGGCCAAGCTGTTGAACTAATGAAGCCACACAAAC
CAGACAGAGAGGATGAAAAGCAGAGAATTGAGGCCCTTGGAGGTTGCGTA
GTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCTGTCGGTTTCCAGAGC
TATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTG
CCTCCACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTGAT
GGCTTCTATGACACCGTGAACCCTGATGAGGCAGTGAAAGTTGTGTCCGA
CCACCTGAAAGAGAATAATGGAGACAGCAGCATGGTTGCCCACAAATTAG
TGGCATCAGCTCGTGATGCTGGGTCAAGTGATAACATCACGGTTATTGTG
GTATTCCTGAGGGACATGAACAAAGCTGTAAATGTTAGTGAGGAATCAGA
TTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGGATGATA
AGGAGAATCATGGAGAGTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCA
GCACCAGCCGACCTAGGCTATGATGGGCGTGTGGATTCATTCACTGATAG
AACTAGCCTGAGCCCAGGGTCCCAAATCAACGTGCTGGAAGACCCAGGCT
ACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTGCCCAGTTT
TTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAGAAAGCAAATCT
TATTAATGAGTTAATGATGGAAAAAAAATCAGTTCAGTCATCATTGCCTG
AATGGAGTGGTGCTGGAGAGTTTCCCACTGCTTTCAATTTGGGTTCAACA
GGGGAGCAGATATACAGAATGCAGAGCTTGTCTCCTGTCTGTTCAGGGTT
GGAAAATGAACAGTTCAAATCCCCGGGAAACAGAGTTTCTAGATTGTCTC
ATTTACGCCACCACTACTCAAAGAAGTGGCACAGATTCAGGTTTAATCCA
AAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCACAAAATAGGCAC
TAGCCTGTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGTT
CTCTGCCATGGAGGCAAAATAGTTGGAAAGGGTACAGTGAAAACATGAGG
AAGCTCAGAAAGACTCATGATATTCCATGCCCAGATCTTCCTTGGAGCTA
TAAAATAGAATAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMKSFRVIFLHIIS
QRKRMAQKGLWDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPH
DPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDS
QVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVS
RAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVV
SDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEE
SDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFT
DRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKA
NLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCS
GLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKI
GTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPW
SYKIE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12199
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 85436 Daltons