cDNA Clone SHIP2

• Plasmid: pCMV-HA SHIP2 Y986F
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-SHIP2 Y986F
• Genbank: CAA74743.1
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGCCTCGGCCTGCGGGGCGCCGGGCCCGGGGGGCGC
CCTGGGCAGCCAGGCCCCCTCCTGGTACCACCGCGACCTGAGCCGGGCGG
CCGCGGAGGAGCTGCTGGCCCGGGCGGGCCGCGATGGCAGCTTCCTGGTC
CGAGACAGCGAGAGCGTGGCGGGGGCCTTCGCGCTCTGCGTCCTGTATCA
GAAGCATGTGCACACGTATCGCATTCTGCCTGATGGAGAAGATTTCTTGG
CTGTGCAGACCTCGCAGGGTGTGCCTGTGCGCCGCTTCCAGACCCTGGGT
GAGCTCATCGGCCTGTACGCCCAGCCCAACCAGGGCCTTGTGTGCGCCCT
GCTTCTTCCTGTAGAGGGTGAGCGAGAGCCGGACCCACCGGATGACCGGG
ATGCCTCAGATGGGGAGGATGAGAAGCCCCCGCTGCCCCCGCGCTCTGGC
TCCACCAGCATTTCTGCCCCCACTGGGCCCAGCAGTCCCCTGCCAGCTCC
TGAGACTCCCACAGCTCCAGCTGCTGAGAGTGCTCCCAATGGGCTGAGCA
CCGTCTCGCACGACTACCTGAAAGGCAGCTATGGGCTGGACCTGGAAGCT
GTGAGGGGTGGAGCCAGCCACCTGCCCCACCTCACCCGTACCCTCGCTAC
CTCATGCCGGAGGCTGCACAGTGAGGTGGACAAGGTCCTGTCAGGCCTGG
AGATCCTGTCCAAGGTGTTTGACCAGCAGAGCTCGCCCATGGTGACCCGC
CTTTTGCAGCAGCAGAACCTGCCACAGACAGGGGAGCAGGAACTAGAGAG
CCTGGTGCTGAAGCTGTCAGTGCTAAAGGACTTCCTGTCAGGCATCCAGA
AGAAGGCCCTGAAGGCCCTACAGGACATGAGCTCCACAGCACCCCCAGCT
CCGCAGCCATCCACACGTAAGGCCAAGACCATCCCCGTGCAGGCCTTTGA
GGTGAAGCTAGATGTGACCCTGGGTGACCTGACCAAGATTGGGAAGTCAC
AGAAGTTCACGCTGAGCGTGGATGTGGAGGGTGGGCGGCTGGTGCTGCTG
CGGAGACAGCGGGACTCCCAGGAGGACTGGACCACCTTCACGCACGACCG
CATCCGCCAGCTCATTAAGTCCCAGCGTGTCCAGAACAAGCTGGGTGTTG
TGTTTGAGAAGGAGAAGGACCGGACTCAGCGCAAGGACTTCATCTTTGTC
AGTGCCCGGAAGCGGGAGGCCTTCTGCCAGCTGTTGCAGCTCATGAAGAA
CAAGCACTCCAAGCAGGACGAGCCCGACATGATCTCAGTCTTCATAGGCA
CCTGGAACATGGGAAGTGTACCACCTCCAAAAAACGTGACATCCTGGTTC
ACATCGAAGGGTCTGGGGAAGACCCTGGACGAGGTCACAGTGACCATACC
CCATGACATCTATGTCTTTGGGACCCAGGAGAACTCAGTGGGCGACCGCG
AGTGGCTGGACCTACTGCGCGGGGGCCTCAAGGAGCTTACGGATCTGGAT
TACCGCCCGATTGCCATGCAATCACTGTGGAATATCAAGGTGGCAGTGCT
GGTCAAGCCAGAGCACGAGAACCGTATCAGCCATGTCAGTACGTCCAGTG
TGAAGACTGGCATCGCCAACACCCTGGGGAACAAGGGGGCTGTGGGCGTC
TCCTTCATGTTTAATGGCACCTCATTTGGCTTTGTGAATTGTCACCTCAC
CTCGGGAAATGAGAAGACGGCTCGGAGGAACCAAAACTACTTGGACATCC
TGCGGCTGCTCTCGCTGGGCGACCGGCAGCTCAATGCCTTTGACATCTCT
CTGCGTTTCACACACCTCTTCTGGTTTGGGGACCTCAACTACCGCCTGGA
CATGGATATCCAGGAGATCCTGAACTACATCAGCAGGAAAGAGTTTGAGC
CCCTCCTCAGGGTGGACCAGCTCAACCTGGAGCGGGAGAAGCACAAGGTC
TTCCTTCGATTCAGTGAGGAGGAGATCTCCTTCCCACCCACCTACCGCTA
TGAGCGGGGTTCCCGGGACACATATGCCTGGCACAAGCAGAAGCCAACTG
GGGTCCGGACCAATGTGCCCTCATGGTGTGACCGGATTCTGTGGAAATCC
TACCCTGAAACTCACATCATCTGCAATTCTTATGGTTGCACTGATGACAT
CGTCACCAGCGACCATTCCCCCGTGTTTGGGACATTTGAGGTTGGAGTTA
CCTCCCAGTTCATCTCCAAGAAAGGGCTCTCAAAGACTTCAGACCAGGCC
TACATTGAGTTTGAGAGCATCGAGGCCATTGTGAAGACAGCCAGCCGCAC
CAAGTTCTTCATCGAGTTCTACTCTACCTGCCTGGAGGAATACAAGAAGA
GCTTTGAGAATGATGCCCAGAGCAGTGACAACATCAACTTCCTCAAAGTG
CAGTGGTCTTCACGCCAGCTGCCCACGCTCAAACCAATTCTGGCTGATAT
CGAGTACCTGCAGGACCAGCACCTCCTGCTCACAGTCAAGTCCATGGATG
GCTATGAATCCTATGGGGAGTGTGTGGTTGCACTCAAATCCATGATCGGC
AGCACGGCCCAACAGTTCCTGACCTTCCTATCCCACCGTGGCGAGGAGAC
AGGCAATATCAGAGGCTCCATGAAGGTGCGGGTGCCCACGGAGCGCCTGG
GCACCCGTGAGCGGCTCTACGAGTGGATCAGCATTGATAAGGATGAGGCA
GGAGCAAAGAGCAAAGCCCCCTCTGTGTCCCGAGGGAGCCAGGAGCCCAG
GTCAGGGAGCCGCAAGCCAGCCTTCACAGAGGCCTCCTGCCCGCTCTCCA
GGTTATTTGAAGAACCAGAGAAACCGCCACCAACGGGGAGGCCCCCAGCC
CCACCCCGAGCAGCTCCCCGGGAGGAGCCCTTGACCCCCAGGTTGAAGCC
AGAGGGAGCTCCTGAACCAGAAGGGGTGGCGGCCCCCCCACCCAAGAACA
GCTTCAATAACCCTGCCTTCTACGTCCTTGAAGGGGTCCCGCACCAGCTG
CTGCCCCCGGAGCCACCCTCGCCTGCCAGGGCCCCTGTCCCATCTGCCAC
CAAGAACAAAGTGGCCATTACAGTGCCTGCTCCACAGCTTGGGCACCACC
GGCACCCTCGTGTGGGAGAGGGGAGTTCTTCAGATGAGGAGTCTGGAGGC
ACACTGCCCCCTCCAGACTTTCCACCTCCACCACTGCCGGACTCAGCCAT
CTTCCTGCCCCCCAGCCTGGATCCTTTACCAGGGCCAGTGGTCCGGGGCC
GTGGTGGGGCTGAGGCCCGTGGCCCACCACCTCCCAAGGCCCATCCAAGG
CCTCCACTGCCCCCAGGCCCCTCACCAGCCAGCACTTTCCTGGGGGAAGT
GGCCAGTGGGGATGACCGGTCCTGCTCGGTGCTGCAGATGGCCAAGACGC
TGAGCGAGGTGGACTATGCCCCTGCTGGGCCTGCACGCTCAGCGCTCCTC
CCAGGCCCCCTGGAGCTGCAGCCCCCCCGGGGACTGCCCTCGGACTATGG
CCGGCCCCTCAGCTTCCCTCCACCCCGCATCCGGGAGAGCATCCAGGAAG
ACCTGGCAGAGGAGGCTCCGTGCCTGCAGGGCGGGCGGGCCAGCGGGCTG
GGCGAGGCAGGCATGAGTGCCTGGCTGCGGGCCATCGGCTTGGAGCGCTA
TGAGGAGGGCCTGGTGCATAATGGCTGGGACGACCTGGAGTTTCTCAGTG
ACATCACCGAGGAGGACTTGGAGGAGGCTGGGGTGCAGGACCCGGCTCAC
AAGCGCCTCCTTCTGGACACCCTGCAGCTCAGCAAGTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYAGSPEFATMASACGAPGPGGALGSQAPSWYHRDLSRAAAEE
LLARAGRDGSFLVRDSESVAGAFALCVLYQKHVHTYRILPDGEDFLAVQT
SQGVPVRRFQTLGELIGLYAQPNQGLVCALLLPVEGEREPDPPDDRDASD
GEDEKPPLPPRSGSTSISAPTGPSSPLPAPETPTAPAAESAPNGLSTVSH
DYLKGSYGLDLEAVRGGASHLPHLTRTLATSCRRLHSEVDKVLSGLEILS
KVFDQQSSPMVTRLLQQQNLPQTGEQELESLVLKLSVLKDFLSGIQKKAL
KALQDMSSTAPPAPQPSTRKAKTIPVQAFEVKLDVTLGDLTKIGKSQKFT
LSVDVEGGRLVLLRRQRDSQEDWTTFTHDRIRQLIKSQRVQNKLGVVFEK
EKDRTQRKDFIFVSARKREAFCQLLQLMKNKHSKQDEPDMISVFIGTWNM
GSVPPPKNVTSWFTSKGLGKTLDEVTVTIPHDIYVFGTQENSVGDREWLD
LLRGGLKELTDLDYRPIAMQSLWNIKVAVLVKPEHENRISHVSTSSVKTG
IANTLGNKGAVGVSFMFNGTSFGFVNCHLTSGNEKTARRNQNYLDILRLL
SLGDRQLNAFDISLRFTHLFWFGDLNYRLDMDIQEILNYISRKEFEPLLR
VDQLNLEREKHKVFLRFSEEEISFPPTYRYERGSRDTYAWHKQKPTGVRT
NVPSWCDRILWKSYPETHIICNSYGCTDDIVTSDHSPVFGTFEVGVTSQF
ISKKGLSKTSDQAYIEFESIEAIVKTASRTKFFIEFYSTCLEEYKKSFEN
DAQSSDNINFLKVQWSSRQLPTLKPILADIEYLQDQHLLLTVKSMDGYES
YGECVVALKSMIGSTAQQFLTFLSHRGEETGNIRGSMKVRVPTERLGTRE
RLYEWISIDKDEAGAKSKAPSVSRGSQEPRSGSRKPAFTEASCPLSRLFE
EPEKPPPTGRPPAPPRAAPREEPLTPRLKPEGAPEPEGVAAPPPKNSFNN
PAFYVLEGVPHQLLPPEPPSPARAPVPSATKNKVAITVPAPQLGHHRHPR
VGEGSSSDEESGGTLPPPDFPPPPLPDSAIFLPPSLDPLPGPVVRGRGGA
EARGPPPPKAHPRPPLPPGPSPASTFLGEVASGDDRSCSVLQMAKTLSEV
DYAPAGPARSALLPGPLELQPPRGLPSDYGRPLSFPPPRIRESIQEDLAE
EAPCLQGGRASGLGEAGMSAWLRAIGLERYEEGLVHNGWDDLEFLSDITE
EDLEEAGVQDPAHKRLLLDTLQLSK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12067
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 140483 Daltons