cDNA Clone PPM1a

• Plasmid: pEBG6P-PPM1a
• Parent Plasmid: pEBG6P
• Expressed: GST-PPM1a
• Genbank: AAH63243.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGAGCATTTTTAGACAAGCCAAAGATGGAAAAGCATAATGC
CCAGGGGCAGGGTAATGGGTTGCGATATGGGCTAAGCAGCATGCAAGGCT
GGCGTGTTGAAATGGAGGATGCACATACGGCTGTGATCGGTTTGCCAAGT
GGACTTGAATCGTGGTCATTCTTTGCTGTGTATGATGGGCATGCTGGTTC
TCAGGTTGCCAAATACTGCTGTGAGCATTTGTTAGATCACATCACCAATA
ACCAGGATTTTAAAGGGTCTGCAGGAGCACCTTCTGTGGAAAATGTAAAG
AATGGAATCAGAACAGGTTTTCTGGAGATTGATGAACACATGAGAGTTAT
GTCAGAGAAGAAACATGGTGCAGATAGAAGTGGGTCAACAGCTGTAGGTG
TCTTAATTTCTCCCCAACATACTTATTTCATTAACTGTGGAGACTCAAGA
GGTTTACTTTGTAGGAACAGGAAAGTTCATTTCTTCACACAAGATCACAA
ACCAAGTAATCCGCTGGAGAAAGAACGAATTCAGAATGCAGGTGGCTCTG
TAATGATTCAGCGTGTGAATGGCTCTCTGGCTGTATCGAGGGCCCTTGGG
GATTTTGATTACAAATGTGTCCATGGAAAAGGTCCTACTGAGCAGCTTGT
CTCACCAGAGCCTGAAGTCCATGATATTGAAAGATCTGAAGAAGATGATC
AGTTCATTATCCTTGCATGTGATGGTATCTGGGATGTTATGGGAAATGAA
GAGCTCTGTGATTTTGTAAGATCCAGACTTGAAGTCACTGATGACCTTGA
GAAAGTTTGCAATGAAGTAGTCGACACCTGTTTGTATAAGGGAAGTCGAG
ACAACATGAGTGTGATTTTGATCTGTTTTCCAAATGCACCCAAAGTATCG
CCAGAAGCAGTGAAGAAGGAGGCAGAGTTGGACAAGTACCTGGAATGCAG
AGTAGAAGAAATCATAAAGAAGCAGGGGGAAGGCGTCCCCGACTTAGTCC
ATGTGATGCGCACATTAGCGAGTGAGAACATCCCCAGCCTCCCACCAGGG
GGTGAATTGGCAAGCAAGAGGAATGTTATTGAAGCCGTTTACAATAGACT
GAATCCTTACAAAAATGACGACACTGACTCTACATCAACAGATGATATGT
GGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMGAFLDKPKMEKH
NAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHA
GSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMR
VMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQD
HKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQ
LVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDD
LEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLE
CRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYN
RLNPYKNDDTDSTSTDDMW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12041
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 69863 Daltons