cDNA Clone TRIF

• Plasmid: pGEX6P-1-TRIF
• Parent Plasmid: pGEX6P2
• Expressed: GST-TRIF
• Genbank: BAC44839.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCTGCACAGGCCCATCACTTCCTAGCGCCTTCGACAT
TCTAGGTGCAGCAGGCCAGGACAAGCTCTTGTATCTGAAGCACAAACTGA
AGACCCCACGCCCAGGCTGCCAGGGGCAGGACCTCCTGCATGCCATGGTT
CTCCTGAAGCTGGGCCAGGAAACTGAGGCCAGGATCTCTCTAGAGGCATT
GAAGGCCGATGCGGTGGCCCGGCTGGTGGCCCGCCAGTGGGCTGGCGTGG
ACAGCACCGAGGACCCAGAGGAGCCCCCAGATGTGTCCTGGGCTGTGGCC
CGCTTGTACCACCTGCTGGCTGAGGAGAAGCTGTGCCCCGCCTCGCTGCG
GGACGTGGCCTACCAGGAAGCCGTCCGCACCCTCAGCTCCAGGGACGACC
ACCGGCTGGGGGAACTTCAGGATGAGGCCCGAAACCGGTGTGGGTGGGAC
ATTGCTGGGGATCCAGGGAGCATCCGGACGCTCCAGTCCAATCTGGGCTG
CCTCCCACCATCCTCGGCTTTGCCCTCTGGGACCAGGAGCCTCCCACGCC
CCATTGACGGTGTTTCGGACTGGAGCCAAGGGTGCTCCCTGCGATCCACT
GGCAGCCCTGCCTCCCTGGCCAGCAACTTGGAAATCAGCCAGTCCCCTAC
CATGCCCTTCCTCAGCCTGCACCGCAGCCCACATGGGCCCAGCAAGCTCT
GTGACGACCCCCAGGCCAGCTTGGTGCCCGAGCCTGTCCCCGGTGGCTGC
CAGGAGCCTGAGGAGATGAGCTGGCCGCCATCGGGGGAGATTGCCAGCCC
ACCAGAGCTGCCAAGCAGCCCACCTCCTGGGCTTCCCGAAGTGGCCCCAG
ATGCAACCTCCACTGGCCTCCCTGATACCCCCGCAGCTCCAGAAACCAGC
ACCAACTACCCAGTGGAGTGCACCGAGGGGTCTGCAGGCCCCCAGTCTCT
CCCCTTGCCTATTCTGGAGCCGGTCAAAAACCCCTGCTCTGTCAAAGACC
AGACGCCACTCCAACTTTCTGTAGAAGATACCACCTCTCCAAATACCAAG
CCGTGCCCACCTACTCCCACCACCCCAGAAACATCCCCTCCTCCTCCTCC
TCCTCCTCCTTCATCTACTCCTTGTTCAGCTCACCTGACCCCCTCCTCCC
TGTTCCCTTCCTCCCTGGAATCATCATCGGAACAGAAATTCTATAACTTT
GTGATCCTCCACGCCAGGGCAGACGAACACATCGCCCTGCGGGTTCGGGA
GAAGCTGGAGGCCCTTGGCGTGCCCGACGGGGCCACCTTCTGCGAGGATT
TCCAGGTGCCGGGGCGCGGGGAGCTGAGCTGCCTGCAGGACGCCATAGAC
CACTCAGCTTTCATCATCCTACTTCTCACCTCCAACTTCGACTGTCGCCT
GAGCCTGCACCAGGTGAACCAAGCCATGATGAGCAACCTCACGCGACAGG
GGTCGCCAGACTGTGTCATCCCCTTCCTGCCCCTGGAGAGCTCCCCGGCC
CAGCTCAGCTCCGACACGGCCAGCCTGCTCTCCGGGCTGGTGCGGCTGGA
CGAACACTCCCAGATCTTCGCCAGGAAGGTGGCCAACACCTTCAAGCCCC
ACAGGCTTCAGGCCCGAAAGGCCATGTGGAGGAAGGAACAGGACACCCGA
GCCCTGCGGGAACAGAGCCAACACCTGGACGGTGAGCGGATGCAGGCGGC
GGCACTGAACGCAGCCTACTCAGCCTACCTCCAGAGCTACTTGTCCTACC
AGGCACAGATGGAGCAGCTCCAGGTGGCTTTTGGGAGCCACATGTCATTT
GGGACTGGGGCGCCCTATGGGGCTCGAATGCCCTTTGGGGGCCAGGTGCC
CCTGGGAGCCCCGCCACCCTTTCCCACTTGGCCGGGGTGCCCGCAGCCGC
CACCCCTGCACGCATGGCAGGCTGGCACCCCCCCACCGCCCTCCCCACAG
CCAGCAGCCTTTCCACAGTCACTGCCCTTCCCGCAGTCCCCAGCCTTCCC
TACGGCCTCACCCGCACCCCCTCAGAGCCCAGGGCTGCAACCCCTCATTA
TCCACCACGCACAGATGGTACAGCTGGGGCTGAACAACCACATGTGGAAC
CAGAGAGGGTCCCAGGCGCCCGAGGACAAGACGCAGGAGGCAGAATGAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMACTGPSL
PSAFDILGAAGQDKLLYLKHKLKTPRPGCQGQDLLHAMVLLKLGQETEAR
ISLEALKADAVARLVARQWAGVDSTEDPEEPPDVSWAVARLYHLLAEEKL
CPASLRDVAYQEAVRTLSSRDDHRLGELQDEARNRCGWDIAGDPGSIRTL
QSNLGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSPASLASNLE
ISQSPTMPFLSLHRSPHGPSKLCDDPQASLVPEPVPGGCQEPEEMSWPPS
GEIASPPELPSSPPPGLPEVAPDATSTGLPDTPAAPETSTNYPVECTEGS
AGPQSLPLPILEPVKNPCSVKDQTPLQLSVEDTTSPNTKPCPPTPTTPET
SPPPPPPPPSSTPCSAHLTPSSLFPSSLESSSEQKFYNFVILHARADEHI
ALRVREKLEALGVPDGATFCEDFQVPGRGELSCLQDAIDHSAFIILLLTS
NFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFLPLESSPAQLSSDTASLLS
GLVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALREQSQHLDG
ERMQAAALNAAYSAYLQSYLSYQAQMEQLQVAFGSHMSFGTGAPYGARMP
FGGQVPLGAPPPFPTWPGCPQPPPLHAWQAGTPPPPSPQPAAFPQSLPFP
QSPAFPTASPAPPQSPGLQPLIIHHAQMVQLGLNNHMWNQRGSQAPEDKT
QEAE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12038
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 104220 Daltons