cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pEBG6P-TPL2 30-467 D270A
• Parent Plasmid: pEBG6P
• Expressed: GST-TPL2 30-468 D270A
• Genbank: P41279
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAAATCTTTATGCAAGTGAAGAGCCAGCAGTTTATGAACC
CAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCTA
AGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGA
TATGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACAC
TGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAA
ACATGGTCATCACTCCCCAAAATGGACGTTACCAAATAGATTCCGATGTT
CTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGTTCTGATTTTAT
TCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCACAAGATATAAAGACGA
AGAAAAGAATGGCGTGTAAACTGATCCCTGTAGATCAATTTAAGCCATCT
GATGTGGAAATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTA
TGGCGCAGTCCTGTGGGGTGAAACTGTCCATCTCTTTATGGAAGCAGGCG
AGGGAGGGTCTGTTCTGGAGAAACTGGAGAGCTGTGGACCAATGAGAGAA
TTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGACTTGATTTTCT
ACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTTT
TCATGTCCACAAAAGCTGTTTTGGTGGCTTTTGGCCTAAGTGTTCAAATG
ACCGAAGATGTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACAT
GAGCCCAGAGGTCATCCTGTGCAGGGGCCATTCAACCAAAGCAGACATCT
ACAGCCTGGGGGCCACGCTCATCCACATGCAGACGGGCACCCCACCCTGG
GTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTGTACATAATCCA
CAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGGA
TGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCA
AGAGCCGCAGACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGA
TCAGCCACGCTGTACGAGTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGC
TGCTGAGTAGGAAGGAGCTGGAACTTCCTGAGAACATTGCTGATTCTTCG
TGCACAGGAAGCACCGAGGAATCTGAGATGCTCAAGAGGCAACGCTCTCT
CTACATCGACCTCGGCGCTCTGGCCGGCTACTTCAATCTTGTTCGGGGAC
CACCAACGCTTGAATATGGCTGAGAATTCGCAAGGGCGAATTCCAGCACA
CTGGCGGCCGTTACTAGTGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMENLYASEEPAVY
EPSLMTMCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHIS
NTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSD
FIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAE
LYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLD
FLHSKKVIHHDIKPSNIVFMSTKAVLVAFGLSVQMTEDVYFPKDLRGTEI
YMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYI
IHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPPR
EDQPRCTSLDSALLERKRLLSRKELELPENIADSSCTGSTEESEMLKRQR
SLYIDLGALAGYFNLVRGPPTLEYG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8982
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST (kinase dead mutation); ; Estimated MW of expressed protein : 76964 Daltons