cDNA Clone TIE2

• Plasmid: pEBG6P-TIE2
• Parent Plasmid: pEBG6P
• Expressed: GST-TIE2
• Genbank: AAH35514.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTT
GCTCCTTTCTGGAACTGTGGAAGGTGCCATGGACTTGATCTTGATCAATT
CCCTACCTCTTGTATCTGATGCTGAAACATCTCTCACCTGCATTGCCTCT
GGGTGGCGCCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTT
AATGAACCAGCACCAGGATCCGCTGGAAGTTACTCAAGATGTGACCAGAG
AATGGGCTAAAAAAGTTGTCTGGAAGAGAGAAAAGGCTAGTAAGATCAAT
GGTGCTTATTTCTGTGAAGGGCGAGTTCGAGGAGAGGCAATCAGGATACG
AACCATGAAGATGCGTCAACAAGCTTCCTTCCTACCAGCTACTTTAACTA
TGACTGTGGACAAGGGAGATAACGTGAACATATCTTTCAAAAAGGTATTG
ATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCCTTCATCCATTC
AGTGCCCCGGCATGAAGTACCTGATATTCTAGAAGTACACCTGCCTCATG
CTCAGCCCCAGGATGCTGGAGTGTACTCGGCCAGGTATATAGGAGGAAAC
CTCTTCACCTCGGCCTTCACCAGGCTGATAGTCCGGAGATGTGAAGCCCA
GAAGTGGGGACCTGAATGCAACCATCTCTGTACTGCTTGTATGAACAATG
GTGTCTGCCATGAAGATACTGGAGAATGCATTTGCCCTCCTGGGTTTATG
GGAAGGACGTGTGAGAAGGCTTGTGAACTGCACACGTTTGGCAGAACTTG
TAAAGAAAGGTGCAGTGGACAAGAGGGATGCAAGTCTTATGTGTTCTGTC
TCCCTGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAAGGGTCTGCAG
TGCAATGAAGCATGCCACCCTGGTTTTTACGGGCCAGATTGTAAGCTTAG
GTGCAGCTGCAACAATGGGGAGATGTGTGATCGTTTCCAAGGATGTCTCT
GCTCTCCAGGATGGCAGGGGCTCCAGTGTGAGAGAGAAGGCATACCGAGG
ATGACCCCAAAGATAGTGGATTTGCCAGATCATATAGAAGTAAACAGTGG
TAAATTTAATCCCATTTGCAAAGCTTCTGGCTGGCCGCTACCTACTAATG
AAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCATCCAAAAGAC
TTTAACCATACGGATCATTTCTCAGTAGCCATATTCACCATCCACCGGAT
CCTCCCCCCTGACTCAGGAGTTTGGGTCTGCAGTGTGAACACAGTGGCTG
GGATGGTGGAAAAGCCCTTCAACATTTCTGTTAAAGTTCTTCCAAAGCCC
CTGAATGCCCCAAACGTGATTGACACTGGACATAACTTTGCTGTCATCAA
CATCAGCTCTGAGCCTTACTTTGGGGATGGACCAATCAAATCCAAGAAGC
TTCTATACAAACCCGTTAATCACTATGAGGCTTGGCAACATATTCAAGTG
ACAAATGAGATTGTTACACTCAACTATTTGGAACCTCGGACAGAATATGA
ACTCTGTGTGCAACTGGTCCGTCGTGGAGAGGGTGGGGAAGGGCATCCTG
GACCTGTGAGACGCTTCACAACAGCTTCTATCGGACTCCCTCCTCCAAGA
GGTCTAAATCTCCTGCCTAAAAGTCAGACCACTCTAAATTTGACCTGGCA
ACCAATATTTCCAAGCTCGGAAGATGACTTTTATGTTGAAGTGGAGAGAA
GGTCTGTGCAAAAAAGTGATCAGCAGAATATTAAAGTTCCAGGCAACTTG
ACTTCGGTGCTACTTAACAACTTACATCCCAGGGAGCAGTACGTGGTCCG
AGCTAGAGTCAACACCAAGGCCCAGGGGGAATGGAGTGAAGATCTCACTG
CTTGGACCCTTAGTGACATTCTTCCTCCTCAACCAGAAAACATCAAGATT
TCCAACATTACACACTCCTCGGCTGTGATTTCTTGGACAATATTGGATGG
CTATTCTATTTCTTCTATTACTATCCGTTACAAGGTTCAAGGCAAGAATG
AAGACCAGCACGTTGATGTGAAGATAAAGAATGCCACCATCACTCAGTAT
CAGCTCAAGGGCCTAGAGCCTGAAACAGCATACCAGGTGGACATTTTTGC
AGAGAACAACATAGGGTCAAGCAACCCAGCCTTTTCTCATGAACTGGTGA
CCCTCCCAGAATCTCAAGCACCAGCGGACCTCGGAGGGGGGAAGATGCTG
CTTATAGCCATCCTTGGCTCTGCTGGAATGACCTGCCTGACTGTGCTGTT
GGCCTTTCTGATCATATTGCAATTGAAGAGAGCAAATGTGCAAAGGAGAA
TGGCCCAAGCCTTCCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAAC
TCAGGGACTCTGGCCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTAC
AATTTATCCAGTGCTTGACTGGAATGACATCAAATTTCAAGATGTGATTG
GGGAGGGCAATTTTGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGG
TTACGGATGGATGCTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGA
TGATCACAGGGACTTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGAC
ACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTAC
TTGTACCTGGCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCT
TCGCAAGAGCCGTGTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATA
GCACCGCGTCCACACTGTCCTCCCATCATCTCCTTCACTTCGCTGCCGAC
GTGGCCCGGGGCATGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGA
TCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAG
CAGATTTTGGATTGTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATG
GGAAGGCTCCCAGTGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGT
GTACACAACCAACAGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGA
TTGTTAGCTTAGGAGGCACACCCTACTGCGGGATGACTTGTGCAGAACTC
TACGAGAAGCTGCCCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGA
TGATGAGGTGTATGATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATG
AGAGGCCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAG
GAGCGAAAGACCTACGTGAATACCACGCTTTATGAGAAGTTTACTTATGC
AGGAATTGACTGTTCTGCTGAAGAAGCGGCCTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMDSL
ASLVLCGVSLLLSGTVEGAMDLILINSLPLVSDAETSLTCIASGWRPHEP
ITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEG
RVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAV
IYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFT
RLIVRRCEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKA
CELHTFGRTCKERCSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHP
GFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQGLQCEREGIPRMTPKIVD
LPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHF
SVAIFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVI
DTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTL
NYLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPK
SQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNN
LHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSS
AVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEP
ETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMLLIAILGS
AGMTCLTVLLAFLIILQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALN
RKVKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAI
KRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEY
APHGNLLDFLRKSRVLETDPAFAIANSTASTLSSHHLLHFAADVARGMDY
LSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQEVYVKKTMGRLPVRW
MAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQG
YRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVN
TTLYEKFTYAGIDCSAEEAA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8936
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 154018 Daltons