cDNA Clone TIE2

• Plasmid: pFBHTb TIE2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TIE2
• Genbank: AAH35514.2
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTT
GCTCCTTTCTGGAACTGTGGAAGGTGCCATGGACTTGATCTTGATCAATT
CCCTACCTCTTGTATCTGATGCTGAAACATCTCTCACCTGCATTGCCTCT
GGGTGGCGCCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTT
AATGAACCAGCACCAGGATCCGCTGGAAGTTACTCAAGATGTGACCAGAG
AATGGGCTAAAAAAGTTGTCTGGAAGAGAGAAAAGGCTAGTAAGATCAAT
GGTGCTTATTTCTGTGAAGGGCGAGTTCGAGGAGAGGCAATCAGGATACG
AACCATGAAGATGCGTCAACAAGCTTCCTTCCTACCAGCTACTTTAACTA
TGACTGTGGACAAGGGAGATAACGTGAACATATCTTTCAAAAAGGTATTG
ATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCCTTCATCCATTC
AGTGCCCCGGCATGAAGTACCTGATATTCTAGAAGTACACCTGCCTCATG
CTCAGCCCCAGGATGCTGGAGTGTACTCGGCCAGGTATATAGGAGGAAAC
CTCTTCACCTCGGCCTTCACCAGGCTGATAGTCCGGAGATGTGAAGCCCA
GAAGTGGGGACCTGAATGCAACCATCTCTGTACTGCTTGTATGAACAATG
GTGTCTGCCATGAAGATACTGGAGAATGCATTTGCCCTCCTGGGTTTATG
GGAAGGACGTGTGAGAAGGCTTGTGAACTGCACACGTTTGGCAGAACTTG
TAAAGAAAGGTGCAGTGGACAAGAGGGATGCAAGTCTTATGTGTTCTGTC
TCCCTGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAAGGGTCTGCAG
TGCAATGAAGCATGCCACCCTGGTTTTTACGGGCCAGATTGTAAGCTTAG
GTGCAGCTGCAACAATGGGGAGATGTGTGATCGTTTCCAAGGATGTCTCT
GCTCTCCAGGATGGCAGGGGCTCCAGTGTGAGAGAGAAGGCATACCGAGG
ATGACCCCAAAGATAGTGGATTTGCCAGATCATATAGAAGTAAACAGTGG
TAAATTTAATCCCATTTGCAAAGCTTCTGGCTGGCCGCTACCTACTAATG
AAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCATCCAAAAGAC
TTTAACCATACGGATCATTTCTCAGTAGCCATATTCACCATCCACCGGAT
CCTCCCCCCTGACTCAGGAGTTTGGGTCTGCAGTGTGAACACAGTGGCTG
GGATGGTGGAAAAGCCCTTCAACATTTCTGTTAAAGTTCTTCCAAAGCCC
CTGAATGCCCCAAACGTGATTGACACTGGACATAACTTTGCTGTCATCAA
CATCAGCTCTGAGCCTTACTTTGGGGATGGACCAATCAAATCCAAGAAGC
TTCTATACAAACCCGTTAATCACTATGAGGCTTGGCAACATATTCAAGTG
ACAAATGAGATTGTTACACTCAACTATTTGGAACCTCGGACAGAATATGA
ACTCTGTGTGCAACTGGTCCGTCGTGGAGAGGGTGGGGAAGGGCATCCTG
GACCTGTGAGACGCTTCACAACAGCTTCTATCGGACTCCCTCCTCCAAGA
GGTCTAAATCTCCTGCCTAAAAGTCAGACCACTCTAAATTTGACCTGGCA
ACCAATATTTCCAAGCTCGGAAGATGACTTTTATGTTGAAGTGGAGAGAA
GGTCTGTGCAAAAAAGTGATCAGCAGAATATTAAAGTTCCAGGCAACTTG
ACTTCGGTGCTACTTAACAACTTACATCCCAGGGAGCAGTACGTGGTCCG
AGCTAGAGTCAACACCAAGGCCCAGGGGGAATGGAGTGAAGATCTCACTG
CTTGGACCCTTAGTGACATTCTTCCTCCTCAACCAGAAAACATCAAGATT
TCCAACATTACACACTCCTCGGCTGTGATTTCTTGGACAATATTGGATGG
CTATTCTATTTCTTCTATTACTATCCGTTACAAGGTTCAAGGCAAGAATG
AAGACCAGCACGTTGATGTGAAGATAAAGAATGCCACCATCACTCAGTAT
CAGCTCAAGGGCCTAGAGCCTGAAACAGCATACCAGGTGGACATTTTTGC
AGAGAACAACATAGGGTCAAGCAACCCAGCCTTTTCTCATGAACTGGTGA
CCCTCCCAGAATCTCAAGCACCAGCGGACCTCGGAGGGGGGAAGATGCTG
CTTATAGCCATCCTTGGCTCTGCTGGAATGACCTGCCTGACTGTGCTGTT
GGCCTTTCTGATCATATTGCAATTGAAGAGAGCAAATGTGCAAAGGAGAA
TGGCCCAAGCCTTCCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAAC
TCAGGGACTCTGGCCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTAC
AATTTATCCAGTGCTTGACTGGAATGACATCAAATTTCAAGATGTGATTG
GGGAGGGCAATTTTGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGG
TTACGGATGGATGCTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGA
TGATCACAGGGACTTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGAC
ACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTAC
TTGTACCTGGCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCT
TCGCAAGAGCCGTGTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATA
GCACCGCGTCCACACTGTCCTCCCATCATCTCCTTCACTTCGCTGCCGAC
GTGGCCCGGGGCATGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGA
TCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAG
CAGATTTTGGATTGTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATG
GGAAGGCTCCCAGTGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGT
GTACACAACCAACAGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGA
TTGTTAGCTTAGGAGGCACACCCTACTGCGGGATGACTTGTGCAGAACTC
TACGAGAAGCTGCCCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGA
TGATGAGGTGTATGATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATG
AGAGGCCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAG
GAGCGAAAGACCTACGTGAATACCACGCTTTATGAGAAGTTTACTTATGC
AGGAATTGACTGTTCTGCTGAAGAAGCGGCCTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVMDSLASLVLC
GVSLLLSGTVEGAMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRD
FEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEA
IRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGS
FIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRR
CEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTF
GRTCKERCSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPD
CKLRCSCNNGEMCDRFQGCLCSPGWQGLQCEREGIPRMTPKIVDLPDHIE
VNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFT
IHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNF
AVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPR
TEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLN
LTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQ
YVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWT
ILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQV
DIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMLLIAILGSAGMTCL
TVLLAFLIILQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKVKNN
PDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEY
ASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNL
LDFLRKSRVLETDPAFAIANSTASTLSSHHLLHFAADVARGMDYLSQKQF
IHRDLAARNILVGENYVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESL
NYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRLEKP
LNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEK
FTYAGIDCSAEEAA

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8934
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 130412 Daltons