cDNA Clone TIE2

• Plasmid: pCMV-HA-2 TIE2
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-TIE2
• Genbank: AAH35514.2
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTT
GCTCCTTTCTGGAACTGTGGAAGGTGCCATGGACTTGATCTTGATCAATT
CCCTACCTCTTGTATCTGATGCTGAAACATCTCTCACCTGCATTGCCTCT
GGGTGGCGCCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTT
AATGAACCAGCACCAGGATCCGCTGGAAGTTACTCAAGATGTGACCAGAG
AATGGGCTAAAAAAGTTGTCTGGAAGAGAGAAAAGGCTAGTAAGATCAAT
GGTGCTTATTTCTGTGAAGGGCGAGTTCGAGGAGAGGCAATCAGGATACG
AACCATGAAGATGCGTCAACAAGCTTCCTTCCTACCAGCTACTTTAACTA
TGACTGTGGACAAGGGAGATAACGTGAACATATCTTTCAAAAAGGTATTG
ATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCCTTCATCCATTC
AGTGCCCCGGCATGAAGTACCTGATATTCTAGAAGTACACCTGCCTCATG
CTCAGCCCCAGGATGCTGGAGTGTACTCGGCCAGGTATATAGGAGGAAAC
CTCTTCACCTCGGCCTTCACCAGGCTGATAGTCCGGAGATGTGAAGCCCA
GAAGTGGGGACCTGAATGCAACCATCTCTGTACTGCTTGTATGAACAATG
GTGTCTGCCATGAAGATACTGGAGAATGCATTTGCCCTCCTGGGTTTATG
GGAAGGACGTGTGAGAAGGCTTGTGAACTGCACACGTTTGGCAGAACTTG
TAAAGAAAGGTGCAGTGGACAAGAGGGATGCAAGTCTTATGTGTTCTGTC
TCCCTGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAAGGGTCTGCAG
TGCAATGAAGCATGCCACCCTGGTTTTTACGGGCCAGATTGTAAGCTTAG
GTGCAGCTGCAACAATGGGGAGATGTGTGATCGTTTCCAAGGATGTCTCT
GCTCTCCAGGATGGCAGGGGCTCCAGTGTGAGAGAGAAGGCATACCGAGG
ATGACCCCAAAGATAGTGGATTTGCCAGATCATATAGAAGTAAACAGTGG
TAAATTTAATCCCATTTGCAAAGCTTCTGGCTGGCCGCTACCTACTAATG
AAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCATCCAAAAGAC
TTTAACCATACGGATCATTTCTCAGTAGCCATATTCACCATCCACCGGAT
CCTCCCCCCTGACTCAGGAGTTTGGGTCTGCAGTGTGAACACAGTGGCTG
GGATGGTGGAAAAGCCCTTCAACATTTCTGTTAAAGTTCTTCCAAAGCCC
CTGAATGCCCCAAACGTGATTGACACTGGACATAACTTTGCTGTCATCAA
CATCAGCTCTGAGCCTTACTTTGGGGATGGACCAATCAAATCCAAGAAGC
TTCTATACAAACCCGTTAATCACTATGAGGCTTGGCAACATATTCAAGTG
ACAAATGAGATTGTTACACTCAACTATTTGGAACCTCGGACAGAATATGA
ACTCTGTGTGCAACTGGTCCGTCGTGGAGAGGGTGGGGAAGGGCATCCTG
GACCTGTGAGACGCTTCACAACAGCTTCTATCGGACTCCCTCCTCCAAGA
GGTCTAAATCTCCTGCCTAAAAGTCAGACCACTCTAAATTTGACCTGGCA
ACCAATATTTCCAAGCTCGGAAGATGACTTTTATGTTGAAGTGGAGAGAA
GGTCTGTGCAAAAAAGTGATCAGCAGAATATTAAAGTTCCAGGCAACTTG
ACTTCGGTGCTACTTAACAACTTACATCCCAGGGAGCAGTACGTGGTCCG
AGCTAGAGTCAACACCAAGGCCCAGGGGGAATGGAGTGAAGATCTCACTG
CTTGGACCCTTAGTGACATTCTTCCTCCTCAACCAGAAAACATCAAGATT
TCCAACATTACACACTCCTCGGCTGTGATTTCTTGGACAATATTGGATGG
CTATTCTATTTCTTCTATTACTATCCGTTACAAGGTTCAAGGCAAGAATG
AAGACCAGCACGTTGATGTGAAGATAAAGAATGCCACCATCACTCAGTAT
CAGCTCAAGGGCCTAGAGCCTGAAACAGCATACCAGGTGGACATTTTTGC
AGAGAACAACATAGGGTCAAGCAACCCAGCCTTTTCTCATGAACTGGTGA
CCCTCCCAGAATCTCAAGCACCAGCGGACCTCGGAGGGGGGAAGATGCTG
CTTATAGCCATCCTTGGCTCTGCTGGAATGACCTGCCTGACTGTGCTGTT
GGCCTTTCTGATCATATTGCAATTGAAGAGAGCAAATGTGCAAAGGAGAA
TGGCCCAAGCCTTCCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAAC
TCAGGGACTCTGGCCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTAC
AATTTATCCAGTGCTTGACTGGAATGACATCAAATTTCAAGATGTGATTG
GGGAGGGCAATTTTGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGG
TTACGGATGGATGCTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGA
TGATCACAGGGACTTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGAC
ACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTAC
TTGTACCTGGCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCT
TCGCAAGAGCCGTGTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATA
GCACCGCGTCCACACTGTCCTCCCATCATCTCCTTCACTTCGCTGCCGAC
GTGGCCCGGGGCATGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGA
TCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAG
CAGATTTTGGATTGTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATG
GGAAGGCTCCCAGTGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGT
GTACACAACCAACAGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGA
TTGTTAGCTTAGGAGGCACACCCTACTGCGGGATGACTTGTGCAGAACTC
TACGAGAAGCTGCCCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGA
TGATGAGGTGTATGATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATG
AGAGGCCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAG
GAGCGAAAGACCTACGTGAATACCACGCTTTATGAGAAGTTTACTTATGC
AGGAATTGACTGTTCTGCTGAAGAAGCGGCCTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMDSLASLVLCGVSLLLSGTVEGAMDLI
LINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPLEVTQD
VTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPA
TLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVH
LPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNHLCTAC
MNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSGQEGCKSY
VFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEMCDRFQ
GCLCSPGWQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPL
PTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSVN
TVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIK
SKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQLVRRGEGGE
GHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVE
VERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSE
DLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQ
GKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSH
ELVTLPESQAPADLGGGKMLLIAILGSAGMTCLTVLLAFLIILQLKRANV
QRRMAQAFQNVREEPAVQFNSGTLALNRKVKNNPDPTIYPVLDWNDIKFQ
DVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLC
KLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFA
IANSTASTLSSHHLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYV
AKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVL
LWEIVSLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWRE
KPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8911
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 128177 Daltons