cDNA Clone SARP1

• Plasmid: pEBG6P-SARP1
• Parent Plasmid: pEBG6P
• Expressed: GST-SARP1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGGACCGCGGCTACGCAGCCAGCGACTCCTCTGGTGTGAGCGG
CAGTGAAGACGCCAGCTACCGCTTCAGTGGCTTTTGGGAGAGAGAAAGTG
AAGACGAAGGTTTCCGCTGCAGCTTCTGGGAGAGAGCAAGAGAGGACCTT
GGGCCCCGTCCTAGTGACGACGGAGAAGAGGGCCGCTGCCGCTGCAGTGG
CTCGTGGGTGAGAGCAAGTGAAGACCGCCGCAGCATCAGGGGCCTGGACT
CAACTCCTCCCCAGAGTCGGAGGTGTTGCGCCATGCCCGGGGTGGCCAAT
TCAGGCCCCTCCACTTCCTCTAGGGAGACTGCAAACCCCTGTTCCAGGAA
GAAGGTGCATTTTGGCAGCATACATGATGCAGTACGAGCTGGAGATGTAA
AGCAGCTTTCAGAAATAGTGGTACGTGGAGCCAGCATTAATGAACTTGAT
GTTCTCCATAAGTTTACCCCTTTACATTGGGCAGCACATTCTGGAAGTTT
GGAGTGTCTTCATTGGCTGCTCTGGCATGGAGCTGATATCACACACGTAA
CAACGAGAGGTTGGACAGCATCTCACATAGCTGCAATCAGGGGTCAGGAT
GCTTGTGTACAGGCTCTTATAATGAATGGAGCAAATCTGACAGCCCAGGA
TGACCGGGGATGCACTCCTTTACATCTTGCTGCAACTCATGGACATTCTT
TCACTTTACAAATAATGCTCCGAAGTGGAGTGGATCCCAGTGTGACTGAT
AAGAGAGAATGGAGACCTGTGCATTATGCAGCTTTTCATGGGCGGCTTGG
CTGCTTGCAACTTCTTGTTAAATGGGGTTGTAGCATAGAAGATGTGGACT
ACAATGGAAACCTTCCAGTTCACTTAGCAGCCATGGAAGGCCACCTTCAC
TGTTTCAAATTCCTAGTCAGTAGAATGAGCAGTGCGACGCAAGTTTTAAA
AGCTTTCAATGATAATGGAGAAAATGTACTGGATTTGGCCCAGAGGTTCT
TCAAGCAGAACATTTTACAGTTTATCCAGGGGGCTGAGTATGAAGGAAAA
GACCTAGAGGATCAGGAAACTTTAGCATTTCCAGGTCATGTGGCTGCCTT
TAAGGGTGATTTGGGGATGCTTAAGAAATTAGTGGAAGATGGAGTAATCA
ATATTAATGAGCGTGCTGATAATGGATCAACTCCTATGCATAAAGCTGCT
GGACAAGGCCACATAGAGTGTTTGCAGTGGTTAATTAAAATGGGAGCAGA
CAGTAATATTACCAACAAAGCAGGGGAGAGACCCAGTGATGTGGCAAAGA
GGTTTGCCCATTTGGCAGCAGTGAAGCTGTTAGAGGAGCTACAGAAATAT
GATATAGATGACGAAAATGAAATTGATGAAAATGATGTGAAATATTTTAT
AAGACATGGTGTTGAGGGAAGCACTGATGCCAAGGATGATTTATGTCTGA
GTGACTTGGATAAAACAGATGCCAGAATGAGAGCTTACAAGAAAATTGTA
GAATTGAGACACCTCCTGGAAATTGCCGAGAGCAACTATAAACACTTGGG
AGGCATAACAGAAGAAGATTTAAAGCAGAAGAAAGAACAGCTTGAGTCTG
AAAAGACCATCAAAGAACTGCAGGGCCAGCTGGAGTATGAACGACTACGT
AGAGAAAAATTAGAATGTCAGCTTGATGAATATCGAGCAGAAGTTGATCA
ACTCAGGGAAACACTGGAAAAAATTCAAGTCCCAAACTTTGTGGCTATGA
CAGCGCTTCTTGTGAGTCAAACAAAGAGAAGAGGCGAGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAADRGY
AASDSSGVSGSEDASYRFSGFWERESEDEGFRCSFWERAREDLGPRPSDD
GEEGRCRCSGSWVRASEDRRSIRGLDSTPPQSRRCCAMPGVANSGPSTSS
RETANPCSRKKVHFGSIHDAVRAGDVKQLSEIVVRGASINELDVLHKFTP
LHWAAHSGSLECLHWLLWHGADITHVTTRGWTASHIAAIRGQDACVQALI
MNGANLTAQDDRGCTPLHLAATHGHSFTLQIMLRSGVDPSVTDKREWRPV
HYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFKFLVS
RMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQET
LAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIEC
LQWLIKMGADSNITNKAGERPSDVAKRFAHLAAVKLLEELQKYDIDDENE
IDENDVKYFIRHGVEGSTDAKDDLCLSDLDKTDARMRAYKKIVELRHLLE
IAESNYKHLGGITEEDLKQKKEQLESEKTIKELQGQLEYERLRREKLECQ
LDEYRAEVDQLRETLEKIQVPNFVAMTALLVSQTKRRGE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8892
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; ; Estimated MW of expressed protein : 94642 Daltons