cDNA Clone RIPK4

• Plasmid: pGEX6P-2-RIPK4
• Parent Plasmid: pGEX6P1
• Expressed: GST-RIPK4
• Genbank: AAI10618
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGGGCGACGGCGGGACCCCATGGGCCCTGGCGCTGCT
GCGCACCTTCGACGCGGGCGAGTTCACGGGCTGGGAGAAGGTGGGCTCGG
GCGGCTTCGGGCAGGTGTACAAGGTACGCCATGTCCACTGGAAGACCTGG
CTGGCCATCAAGTGCTCGCCCAGCCTGCACGTCGACGACAGGGAGCGCAT
GGAGCTTTTGGAAGAAGCCAAGAAGATGGAGATGGCCAAGTTTCGCTACA
TCCTGCCTGTGTATGGCATCTGCCGCGAACCTGTCGGCCTGGTCATGGAG
TACATGGAGACGGGCTCCCTGGAAAAGCTGCTGGCTTCGGAGCCATTGCC
ATGGGATCTCCGGTTCCGAATCATCCACGAGACGGCGGTGGGCATGAACT
TCCTGCACTGCATGGCCCCGCCACTCCTGCACCTGGACCTCAAGCCCGCG
AACATCCTGCTGGATGCCCACTACCACGTCAAGATTTCTGATTTTGGTCT
GGCCAAGTGCAACGGGCTGTCCCACTCGCATGACCTCAGCATGGATGGCC
TGTTTGGCACAATCGCCTACCTCCCTCCAGAGCGCATCAGGGAGAAGAGC
CGGCTCTTCGACACCAAGCACGATGTATACAGCTTTGCGATCGTCATCTG
GGGCGTGCTCACACAGAAGAAGCCGTTTGCAGATGAGAAGAACATCCTGC
ACATCATGGTGAAGGTGGTGAAGGGCCACCGCCCCGAGCTGCCGCCCGTG
TGCAGAGCCCGGCCGCGCGCCTGCAGCCACCTGATACGCCTCATGCAGCG
GTGCTGGCAGGGGGATCCGCGAGTTAGGCCCACCTTCCAAGAAATTACTT
CTGAAACCGAGGACCTGTGTGAAAAGCCTGATGACGAAGTGAAAGAAACT
GCTCATGATCTGGACGTGAAAAGCCCCCCGGAGCCCAGGAGCGAGGTGGT
GCCTGCGAGGCTCAAGCGGGCCTCTGCCCCCACCTTCGATAACGACTACA
GCCTCTCCGAGCTGCTCTCACAGCTGGACTCTGGAGTTTCCCAGGCTGTC
GAGGGCCCCGAGGAGCTCAGCCGCAGCTCCTCTGAGTCCAAGCTGCCATC
GTCCGGCAGTGGGAAGAGGCTCTCGGGGGTGTCCTCGGTGGACTCCGCCT
TCTCTTCCAGAGGATCACTGTCGCTGTCCTTTGAGCGGGAACCTTCAACC
AGCGATCTGGGCACCACAGACGTCCAGAAGAAGAAGCTTGTGGATGCCAT
CGTGTCCGGGGACACCAGCAAACTGATGAAGATCCTGCAGCCGCAGGACG
TGGACCTGGCACTGGACAGCGGTGCCAGCCTGCTGCACCTGGCGGTGGAG
GCCGGGCAAGAGGAGTGCGCCAAGTGGCTGCTGCTCAACAATGCCAACCC
CAACCTGAGCAACCGTAGGGGCTCCACCCCGTTGCACATGGCCGTGGAGA
GGAGGGTGCGGGGTGTCGTGGAGCTCCTGCTGGCGCGGAAGATCAGTGTC
AACGCCAAGGATGAGGACCAGTGGACAGCCCTCCACTTTGCAGCCCAGAA
CGGGGACGAGTCTAGCACACGGCTGCTGTTGGAGAAGAACGCCTCGGTCA
ACGAGGTGGACTTTGAGGGCCGGACGCCCATGCACGTGGCCTGCCAGCAC
GGGCAGGAGAATATCGTGCGCATCCTGCTGCGCCGAGGCGTGGACGTGAG
CCTGCAGGGCAAGGATGCCTGGCTGCCACTGCACTACGCTGCCTGGCAGG
GCCACCTGCCCATCGTCAAGCTGCTGGCCAAGCAGCCGGGGGTGAGTGTG
AACGCCCAGACGCTGGATGGGAGGACGCCATTGCACCTGGCCGCACAGCG
CGGGCACTACCGCGTGGCCCGCATCCTCATCGACCTGTGCTCCGACGTCA
ACGTCTGCAGCCTGCTGGCACAGACACCCCTGCACGTGGCCGCGGAGACG
GGGCACACGAGCACTGCCAGGCTGCTCCTGCATCGGGGCGCTGGCAAGGA
GGCCGTGACCTCAGACGGCTACACCGCTCTGCACCTGGCTGCCCGCAACG
GACACCTGGCCACTGTCAAGCTGCTTGTCGAGGAGAAGGCCGATGTGCTG
GCCCGGGGACCCCTGAACCAGACGGCGCTGCACCTGGCTGCCGCCCACGG
GCACTCGGAGGTGGTGGAGGAGTTGGTCAGCGCCGATGTCATTGACCTGT
TCGACGAGCAGGGGCTCAGCGCGCTGCACCTGGCCGCCCAGGGCCGGCAC
GCACAGACGGTGGAGACTCTGCTCAGGCATGGGGCCCACATCAACCTGCA
GAGCCTCAAGTTCCAGGGCGGCCATGGCCCCGCCGCCACACTCCTGCGGC
GAAGCAAGACCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMEGDGGTP
WALALLRTFDAGEFTGWEKVGSGGFGQVYKVRHVHWKTWLAIKCSPSLHV
DDRERMELLEEAKKMEMAKFRYILPVYGICREPVGLVMEYMETGSLEKLL
ASEPLPWDLRFRIIHETAVGMNFLHCMAPPLLHLDLKPANILLDAHYHVK
ISDFGLAKCNGLSHSHDLSMDGLFGTIAYLPPERIREKSRLFDTKHDVYS
FAIVIWGVLTQKKPFADEKNILHIMVKVVKGHRPELPPVCRARPRACSHL
IRLMQRCWQGDPRVRPTFQEITSETEDLCEKPDDEVKETAHDLDVKSPPE
PRSEVVPARLKRASAPTFDNDYSLSELLSQLDSGVSQAVEGPEELSRSSS
ESKLPSSGSGKRLSGVSSVDSAFSSRGSLSLSFEREPSTSDLGTTDVQKK
KLVDAIVSGDTSKLMKILQPQDVDLALDSGASLLHLAVEAGQEECAKWLL
LNNANPNLSNRRGSTPLHMAVERRVRGVVELLLARKISVNAKDEDQWTAL
HFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQHGQENIVRILLR
RGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPL
HLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLH
RGAGKEAVTSDGYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALH
LAAAHGHSEVVEELVSADVIDLFDEQGLSALHLAAQGRHAQTVETLLRHG
AHINLQSLKFQGGHGPAATLLRRSKT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8702
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 114207 Daltons