cDNA Clone RIPK5

• Plasmid: pFBHTb RIPK5
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-RIPK5
• Genbank: AAH72406
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGGGCGACGGGGTGCCATGGGGCAGCGAGCCCGTCTCGGG
TCCCGGCCCCGGCGGCGGCGGAATGATCCGCGAGCTGTGCCGGGGCTTCG
GCCGCTACCGCCGCTACCTGGGACGGCTGCGACAGAACCTGCGCGAGACC
CAGAAGTTCTTCCGCGACATCAAGTGCTCCCACAACCACACTTGTCTCTC
CTCCCTCACGGGCGGCGGCGGGGCCGAGCGCGGCCCTGCAGGCGATGTCG
CCGAAACCGGGCTGCAGGCGGGCCAACTGAGCTGCATTTCCTTCCCACCT
AAGGAAGAGAAGTACCTCCAGCAGATTGTGGACTGCCTCCCTTGCATACT
GATCCTCGGCCAGGATTGTAACGTCAAGTGCCAGCTGTTGAATCTGCTGT
TGGGGGTGCAGGTGCTTCCCACCACCAAGCTGGGCAGTGAGGAGAGCTGT
AAGCTTCGGCGCCTCCGCTTCACCTATGGGACTCAGACTCGGGTCAGCCT
GGCGCTCCCTGGACAGTATGAACTAGTGCACACGCTGGTTGCTCATCAGG
GCAACTGGGAGACCATCCCTGAGGAGGATCTGGAGGTCCAAGAGAACAAT
GAGGATGCTGCTCATGTTTTAGCGGAACTGGAGGTAACGATGCACCATGC
TCTCTTACAGGAAGTGGACGTTGTGGTAGCACCATGCCAAGGCCTCCGGC
CCACAGTGGATGTTCTGGGTGACTTGGTGAATGATTTCTTGCCTGCGATA
ACCTATGCACTCCACAAAGATGAACTCTCTGAGAGGGATGAGCAAGAGCT
TCAGGAAATCCGAAAGTATTTCTCCTTTCCTGTATTCTTTTTCAAAGTGC
CGAAACTGGGCTCGGAGATAATAGACTCCTCAACCAGGAGAATGGAGAGC
GAAAGATCACCGCTTTATCGCCAGCTAATTGACCTGGGCTATCTGAGCAG
CAGTCACTGGAACTGTGGGGCTCCTGGCCAGGATACTAAAGCTCAGAGCA
TGTTGGTGGAACAGAGTGAAAAGCTGAGACACTTGAGCACATTTTCTCAC
CAGGTGTTACAGACTCGCCTGGTGGATGCAGCCAAGGCCCTGAACCTGGT
GCACTGCCACTGCCTTGACATCTTTATTAACCAGGCATTTGACATGCAGC
GGGACCTGCAGATCACTCCCAAACGTCTGGAATATACTCGAAAAAAGGAG
AATGAGTTGTATGAATCATTGATGAATATTGCCAACCGAAAGCAGGAGGA
AATGAAGGATATGATTGTTGAGACACTTAATACCATGAAGGAGGAACTTC
TGGATGATGCTACTAACATGGAGTTTAAAGACGTCATTGTCCCTGAGAAT
GGAGAACCAGTAGGCACCAGAGAGATCAAATGCTGCATCCGACAGATCCA
GGAACTCATCATCTCCCGACTTAATCAGGCAGTGGCTAATAAGCTGATCA
GCTCAGTGGATTACCTGAGGGAAAGCTTCGTCGGAACCCTGGAACGATGT
CTGCAGAGCCTGGAGAAGTCTCAGGATGTCTCAGTTCACATCACCAGTAA
TTATCTCAAACAGATCTTAAATGCTGCCTATCATGTTGAAGTCACGTTTC
ACTCAGGGTCGTCAGTTACAAGGATGCTATGGGAGCAAATCAAACAGATC
ATCCAGCGCATCACATGGGTGAGCCCACCTGCCATCACTCTGGAATGGAA
GAGGAAGGTGGCCCAGGAAGCCATTGAGAGCCTCAGCGCCTCCAAATTGG
CTAAGAGCATTTGCAGCCAATTCCGGACTCGGCTCAATAGTTCCCACGAG
GCTTTTGCAGCCTCCTTGCGGCAGCTGGAAGCTGGCCACTCAGGCCGGTT
AGAGAAAACGGAAGATCTATGGCTGAGGGTTCGGAAAGATCATGCTCCCC
GCCTGGCCCGCCTTTCTCTGGAAAGCCGTTCTTTACAGGATGTCTTGCTT
CATCGTAAACCTAAACTGGGACAGGAACTGGGCCGGGGCCAGTATGGTGT
GGTATACCTGTGTGACAACTGGGGAGGACACTTTCCTTGTGCCCTCAAAT
CAGTTGTCCCTCCAGATGAGAAGCACTGGAATGATCTGGCTTTGGAATTT
CACTATATGAGGTCTCTGCCGAAGCATGAGCGATTGGTGGATCTCCATGG
TTCAGTCATTGACTACAACTATGGTGGTGGCTCCAGCATTGCTGTGCTCC
TCATTATGGAGCGGCTACACCGGGATCTCTACACAGGGCTGAAGGCTGGG
CTGACCCTGGAGACACGTTTGCAGATAGCACTAGATGTGGTGGAGGGAAT
CCGCTTCCTGCACAGCCAGGGACTTGTCCATCGTGATATCAAACTGAAAA
ATGTGCTGCTGGATAAGCAGAACCGTGCCAAGATCACTGACTTAGGATTC
TGCAAGCCAGAGGCCATGATGTCAGGCAGCATTGTGGGGACACCAATCCA
TATGGCCCCTGAACTTTTCACAGGGAAGTACGATAATTCCGTGGATGTCT
ACGCTTTTGGAATTCTTTTCTGGTATATCTGCTCAGGCTCTGTCAAGCTC
CCTGAGGCATTTGAGAGGTGTGCTAGCAAAGACCATCTCTGGAACAATGT
GCGGAGGGGGGCTCGCCCAGAACGTCTTCCTGTGTTTGATGAGGAGTGCT
GGCAGTTGATGGAAGCCTGTTGGGATGGCGACCCCTTGAAGAGGCCTCTC
TTGGGCATTGTCCAGCCCATGCTCCAGGGCATCATGAATCGGCTCTGCAA
GTCCAATTCTGAGCAGCCAAACAGAGGACTAGATGATTCTACTTGAGCGG
CCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMEGDGVPWGSEPVSGPGPGGGG
MIRELCRGFGRYRRYLGRLRQNLRETQKFFRDIKCSHNHTCLSSLTGGGG
AERGPAGDVAETGLQAGQLSCISFPPKEEKYLQQIVDCLPCILILGQDCN
VKCQLLNLLLGVQVLPTTKLGSEESCKLRRLRFTYGTQTRVSLALPGQYE
LVHTLVAHQGNWETIPEEDLEVQENNEDAAHVLAELEVTMHHALLQEVDV
VVAPCQGLRPTVDVLGDLVNDFLPAITYALHKDELSERDEQELQEIRKYF
SFPVFFFKVPKLGSEIIDSSTRRMESERSPLYRQLIDLGYLSSSHWNCGA
PGQDTKAQSMLVEQSEKLRHLSTFSHQVLQTRLVDAAKALNLVHCHCLDI
FINQAFDMQRDLQITPKRLEYTRKKENELYESLMNIANRKQEEMKDMIVE
TLNTMKEELLDDATNMEFKDVIVPENGEPVGTREIKCCIRQIQELIISRL
NQAVANKLISSVDYLRESFVGTLERCLQSLEKSQDVSVHITSNYLKQILN
AAYHVEVTFHSGSSVTRMLWEQIKQIIQRITWVSPPAITLEWKRKVAQEA
IESLSASKLAKSICSQFRTRLNSSHEAFAASLRQLEAGHSGRLEKTEDLW
LRVRKDHAPRLARLSLESRSLQDVLLHRKPKLGQELGRGQYGVVYLCDNW
GGHFPCALKSVVPPDEKHWNDLALEFHYMRSLPKHERLVDLHGSVIDYNY
GGGSSIAVLLIMERLHRDLYTGLKAGLTLETRLQIALDVVEGIRFLHSQG
LVHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFT
GKYDNSVDVYAFGILFWYICSGSVKLPEAFERCASKDHLWNNVRRGARPE
RLPVFDEECWQLMEACWDGDPLKRPLLGIVQPMLQGIMNRLCKSNSEQPN
RGLDDST

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8683
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 108599 Daltons