cDNA Clone RIPK5

• Plasmid: pEBG6P-RIPK5
• Parent Plasmid: pEBG6P
• Expressed: GST-RIPK5
• Genbank: AAH72406
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGGGCGACGGGGTGCCATGGGGCAGCGAGCCCGTCTCGGG
TCCCGGCCCCGGCGGCGGCGGAATGATCCGCGAGCTGTGCCGGGGCTTCG
GCCGCTACCGCCGCTACCTGGGACGGCTGCGACAGAACCTGCGCGAGACC
CAGAAGTTCTTCCGCGACATCAAGTGCTCCCACAACCACACTTGTCTCTC
CTCCCTCACGGGCGGCGGCGGGGCCGAGCGCGGCCCTGCAGGCGATGTCG
CCGAAACCGGGCTGCAGGCGGGCCAACTGAGCTGCATTTCCTTCCCACCT
AAGGAAGAGAAGTACCTCCAGCAGATTGTGGACTGCCTCCCTTGCATACT
GATCCTCGGCCAGGATTGTAACGTCAAGTGCCAGCTGTTGAATCTGCTGT
TGGGGGTGCAGGTGCTTCCCACCACCAAGCTGGGCAGTGAGGAGAGCTGT
AAGCTTCGGCGCCTCCGCTTCACCTATGGGACTCAGACTCGGGTCAGCCT
GGCGCTCCCTGGACAGTATGAACTAGTGCACACGCTGGTTGCTCATCAGG
GCAACTGGGAGACCATCCCTGAGGAGGATCTGGAGGTCCAAGAGAACAAT
GAGGATGCTGCTCATGTTTTAGCGGAACTGGAGGTAACGATGCACCATGC
TCTCTTACAGGAAGTGGACGTTGTGGTAGCACCATGCCAAGGCCTCCGGC
CCACAGTGGATGTTCTGGGTGACTTGGTGAATGATTTCTTGCCTGCGATA
ACCTATGCACTCCACAAAGATGAACTCTCTGAGAGGGATGAGCAAGAGCT
TCAGGAAATCCGAAAGTATTTCTCCTTTCCTGTATTCTTTTTCAAAGTGC
CGAAACTGGGCTCGGAGATAATAGACTCCTCAACCAGGAGAATGGAGAGC
GAAAGATCACCGCTTTATCGCCAGCTAATTGACCTGGGCTATCTGAGCAG
CAGTCACTGGAACTGTGGGGCTCCTGGCCAGGATACTAAAGCTCAGAGCA
TGTTGGTGGAACAGAGTGAAAAGCTGAGACACTTGAGCACATTTTCTCAC
CAGGTGTTACAGACTCGCCTGGTGGATGCAGCCAAGGCCCTGAACCTGGT
GCACTGCCACTGCCTTGACATCTTTATTAACCAGGCATTTGACATGCAGC
GGGACCTGCAGATCACTCCCAAACGTCTGGAATATACTCGAAAAAAGGAG
AATGAGTTGTATGAATCATTGATGAATATTGCCAACCGAAAGCAGGAGGA
AATGAAGGATATGATTGTTGAGACACTTAATACCATGAAGGAGGAACTTC
TGGATGATGCTACTAACATGGAGTTTAAAGACGTCATTGTCCCTGAGAAT
GGAGAACCAGTAGGCACCAGAGAGATCAAATGCTGCATCCGACAGATCCA
GGAACTCATCATCTCCCGACTTAATCAGGCAGTGGCTAATAAGCTGATCA
GCTCAGTGGATTACCTGAGGGAAAGCTTCGTCGGAACCCTGGAACGATGT
CTGCAGAGCCTGGAGAAGTCTCAGGATGTCTCAGTTCACATCACCAGTAA
TTATCTCAAACAGATCTTAAATGCTGCCTATCATGTTGAAGTCACGTTTC
ACTCAGGGTCGTCAGTTACAAGGATGCTATGGGAGCAAATCAAACAGATC
ATCCAGCGCATCACATGGGTGAGCCCACCTGCCATCACTCTGGAATGGAA
GAGGAAGGTGGCCCAGGAAGCCATTGAGAGCCTCAGCGCCTCCAAATTGG
CTAAGAGCATTTGCAGCCAATTCCGGACTCGGCTCAATAGTTCCCACGAG
GCTTTTGCAGCCTCCTTGCGGCAGCTGGAAGCTGGCCACTCAGGCCGGTT
AGAGAAAACGGAAGATCTATGGCTGAGGGTTCGGAAAGATCATGCTCCCC
GCCTGGCCCGCCTTTCTCTGGAAAGCCGTTCTTTACAGGATGTCTTGCTT
CATCGTAAACCTAAACTGGGACAGGAACTGGGCCGGGGCCAGTATGGTGT
GGTATACCTGTGTGACAACTGGGGAGGACACTTTCCTTGTGCCCTCAAAT
CAGTTGTCCCTCCAGATGAGAAGCACTGGAATGATCTGGCTTTGGAATTT
CACTATATGAGGTCTCTGCCGAAGCATGAGCGATTGGTGGATCTCCATGG
TTCAGTCATTGACTACAACTATGGTGGTGGCTCCAGCATTGCTGTGCTCC
TCATTATGGAGCGGCTACACCGGGATCTCTACACAGGGCTGAAGGCTGGG
CTGACCCTGGAGACACGTTTGCAGATAGCACTAGATGTGGTGGAGGGAAT
CCGCTTCCTGCACAGCCAGGGACTTGTCCATCGTGATATCAAACTGAAAA
ATGTGCTGCTGGATAAGCAGAACCGTGCCAAGATCACTGACTTAGGATTC
TGCAAGCCAGAGGCCATGATGTCAGGCAGCATTGTGGGGACACCAATCCA
TATGGCCCCTGAACTTTTCACAGGGAAGTACGATAATTCCGTGGATGTCT
ACGCTTTTGGAATTCTTTTCTGGTATATCTGCTCAGGCTCTGTCAAGCTC
CCTGAGGCATTTGAGAGGTGTGCTAGCAAAGACCATCTCTGGAACAATGT
GCGGAGGGGGGCTCGCCCAGAACGTCTTCCTGTGTTTGATGAGGAGTGCT
GGCAGTTGATGGAAGCCTGTTGGGATGGCGACCCCTTGAAGAGGCCTCTC
TTGGGCATTGTCCAGCCCATGCTCCAGGGCATCATGAATCGGCTCTGCAA
GTCCAATTCTGAGCAGCCAAACAGAGGACTAGATGATTCTACTTGAGCGG
CCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEGDGVPWGSEPV
SGPGPGGGGMIRELCRGFGRYRRYLGRLRQNLRETQKFFRDIKCSHNHTC
LSSLTGGGGAERGPAGDVAETGLQAGQLSCISFPPKEEKYLQQIVDCLPC
ILILGQDCNVKCQLLNLLLGVQVLPTTKLGSEESCKLRRLRFTYGTQTRV
SLALPGQYELVHTLVAHQGNWETIPEEDLEVQENNEDAAHVLAELEVTMH
HALLQEVDVVVAPCQGLRPTVDVLGDLVNDFLPAITYALHKDELSERDEQ
ELQEIRKYFSFPVFFFKVPKLGSEIIDSSTRRMESERSPLYRQLIDLGYL
SSSHWNCGAPGQDTKAQSMLVEQSEKLRHLSTFSHQVLQTRLVDAAKALN
LVHCHCLDIFINQAFDMQRDLQITPKRLEYTRKKENELYESLMNIANRKQ
EEMKDMIVETLNTMKEELLDDATNMEFKDVIVPENGEPVGTREIKCCIRQ
IQELIISRLNQAVANKLISSVDYLRESFVGTLERCLQSLEKSQDVSVHIT
SNYLKQILNAAYHVEVTFHSGSSVTRMLWEQIKQIIQRITWVSPPAITLE
WKRKVAQEAIESLSASKLAKSICSQFRTRLNSSHEAFAASLRQLEAGHSG
RLEKTEDLWLRVRKDHAPRLARLSLESRSLQDVLLHRKPKLGQELGRGQY
GVVYLCDNWGGHFPCALKSVVPPDEKHWNDLALEFHYMRSLPKHERLVDL
HGSVIDYNYGGGSSIAVLLIMERLHRDLYTGLKAGLTLETRLQIALDVVE
GIRFLHSQGLVHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTP
IHMAPELFTGKYDNSVDVYAFGILFWYICSGSVKLPEAFERCASKDHLWN
NVRRGARPERLPVFDEECWQLMEACWDGDPLKRPLLGIVQPMLQGIMNRL
CKSNSEQPNRGLDDST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8681
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 132645 Daltons