cDNA Clone TNF alpha

• Plasmid: pFBHTb TNFalpha 77-234
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TNFalpha 77-234
• Genbank: AAH28148.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGTCAGATCATCTTCTCGAACCCCGAGTGACAAGCCTGTAGCCCA
TGTTGTAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCC
GGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTG
GTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAA
GGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATCAGCC
GCATCGCCGTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCCATCAAG
AGCCCCTGCCAGAGGGAGACCCCAGAGGGGGCTGAGGCCAAGCCCTGGTA
TGAGCCCATCTATCTGGGAGGGGTCTTCCAGCTGGAGAAGGGTGACCGAC
TCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTTGCCGAGTCTGGG
CAGGTCTACTTTGGGATCATTGCCCTGTGAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSVRSSSRTPSDKPVAHVVANPQA
EGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPST
HVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGG
VFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 20723 Daltons