cDNA Clone RIPK3

• Plasmid: pGEX6P-2-RIPK3
• Parent Plasmid: pGEX6P2
• Expressed: GST-RIPK3
• Genbank: AAH62584
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCGTGCGTCAAGTTATGGCCCAGCGGTGCCCCCGCCCC
CTTGGTGTCCATCGAGGAACTGGAGAACCAGGAGCTCGTCGGCAAAGGCG
GGTTCGGCACAGTGTTCCGGGCGCAACATAGGAAGTGGGGCTACGATGTG
GCGGTCAAGATCGTAAACTCGAAGGCGATATCCAGGGAGGTCAAGGCCAT
GGCAAGTCTGGATAACGAATTCGTGCTGCGCCTAGAAGGGGTTATCGAGA
AGGTGAACTGGGACCAAGATCCCAAGCCGGCTCTGGTGACTAAATTCATG
GAGAACGGCTCCTTGTCGGGGCTGCTGCAGTCCCAGTGCCCTCGGCCCTG
GCCGCTCCTTTGCCGCCTGCTGAAAGAAGTGGTGCTTGGGATGTTTTACC
TGCACGACCAGAACCCGGTGCTCCTGCACCGGGACCTCAAGCCATCCAAC
GTCCTGCTGGACCCAGAGCTGCACGTCAAGCTGGCAGATTTTGGCCTGTC
CACATTTCAGGGAGGCTCACAGTCAGGGACAGGGTCCGGGGAGCCAGGGG
GCACCCTGGGCTACTTGGCCCCAGAACTGTTTGTTAACGTAAACCGGAAG
GCCTCCACAGCCAGTGACGTCTACAGCTTCGGGATCCTAATGTGGGCAGT
GCTTGCTGGAAGAGAAGTTGAGTTGCCAACCGAACCATCACTCGTGTACG
AAGCAGTGTGCAACAGGCAGAACCGGCCTTCATTGGCTGAGCTGCCCCAA
GCCGGGCCTGAGACTCCCGGCTTAGAAGGACTGAAGGAGCTAATGCAGCT
CTGCTGGAGCAGTGAGCCCAAGGACAGACCCTCCTTCCAGGAATGCCTAC
CAAAAACTGATGAAGTCTTCCAGATGGTGGAGAACAATATGAATGCTGCT
GTCTCCACGGTAAAGGATTTCCTGTCTCAGCTCAGGAGCAGCAATAGGAG
ATTTTCTATCCCAGAGTCAGGCCAAGGAGGGACAGAAATGGATGGCTTTA
GGAGAACCATAGAAAACCAGCACTCTCGTAATGATGTCATGGTTTCTGAG
TGGCTAAACAAACTGAATCTAGAGGAGCCTCCCAGCTCTGTTCCTAAAAA
ATGCCCGAGCCTTACCAAGAGGAGCAGGGCACAAGAGGAGCAGGTTCCAC
AAGCCTGGACAGCAGGCACATCTTCAGATTCGATGGCCCAACCTCCCCAG
ACTCCAGAGACCTCAACTTTCAGAAACCAGATGCCCAGCCCTACCTCAAC
TGGAACACCAAGTCCTGGACCCCGAGGGAATCAGGGGGCTGAGAGACAAG
GCATGAACTGGTCCTGCAGGACCCCGGAGCCAAATCCAGTAACAGGGCGA
CCGCTCGTTAACATATACAACTGCTCTGGGGTGCAAGTTGGAGACAACAA
CTACTTGACTATGCAACAGACAACTGCCTTGCCCACATGGGGCTTGGCAC
CTTCGGGCAAGGGGAGGGGCTTGCAGCACCCCCCACCAGTAGGTTCGCAA
GAAGGCCCTAAAGATCCTGAAGCCTGGAGCAGGCCACAGGGTTGGTATAA
TCATAGCGGGAAATAAGCGGCCGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAMSCVKLWPS
GAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISR
EVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQ
CPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLA
DFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGI
LMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLK
ELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLR
SSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPS
SVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMP
SPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQ
VGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRP
QGWYNHSGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8630
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 84617 Daltons