cDNA Clone RIPK2

• Plasmid: pCMV-FLAG RIPK2 T412A
• Parent Plasmid: pCMV5
• Expressed: FLAG-RIPK2 T412A
• Genbank: NM_003821
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGATGAACGGGGA
GGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGACC
TGCGCTACCTGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCAC
GCAGACTGGCGCGTCCAGGTGGCCGTGAAGCACCTGCACATCCACACTCC
GCTGCTCGACAGTGAAAGAAAGGATGTCTTAAGAGAAGCTGAAATTTTAC
ACAAAGCTAGATTTAGTTACATTCTTCCAATTTTGGGAATTTGCAATGAG
CCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAATGGATCATTAAA
TGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGCTTGGCCATTGA
GATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATTACCTGCACAAT
ATGACTCCTCCTTTACTTCATCATGACTTGAAGACTCAGAATATCTTATT
GGACAATGAATTTCATGTTAAGATTGCAGATTTTGGTTTATCAAAGTGGC
GCATGATGTCCCTCTCACAGTCACGAAGTAGCAAATCTGCACCAGAAGGA
GGGACAATTATCTATATGCCACCTGAAAACTATGAACCTGGACAAAAATC
AAGGGCCAGTATCAAGCACGATATATATAGCTATGCAGTTATCACATGGG
AAGTGTTATCCAGAAAACAGCCTTTTGAAGATGTCACCAATCCTTTGCAG
ATAATGTATAGTGTGTCACAAGGACATCGACCTGTTATTAATGAAGAAAG
TTTGCCATATGATATACCTCACCGAGCACGTATGATCTCTCTAATAGAAA
GTGGATGGGCACAAAATCCAGATGAAAGACCATCTTTCTTAAAATGTTTA
ATAGAACTTGAACCAGTTTTGAGAACATTTGAAGAGATAACTTTTCTTGA
AGCTGTTATTCAGCTAAAGAAAACAAAGTTACAGAGTGTTTCAAGTGCCA
TTCACCTATGTGACAAGAAGAAAATGGAATTATCTCTGAACATACCTGTA
AATCATGGTCCACAAGAGGAATCATGTGGATCCTCTCAGCTCCATGAAAA
TAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCCAGCTCCTCAAGACAATG
ATTTTTTATCTAGAAAAGCTCAAGACTGTTATTTTATGAAGCTGCATCAC
TGTCCTGGAAATCACAGTTGGGATAGCACCATTTCTGGATCTCAAAGGGC
TGCATTCTGTGATCACAAGACCGCTCCATGCTCTTCAGCAATAATAAATC
CACTCTCAACTGCAGGAAACTCAGAACGTCTGCAGCCTGGTATAGCCCAG
CAGTGGATCCAGAGCAAAAGGGAAGACATTGTGAACCAAATGACAGAAGC
CTGCCTTAACCAGTCGCTAGATGCCCTTCTGTCCAGGGACTTGATCATGA
AAGAGGACTATGAACTTGTTAGTACCAAGCCTACAAGGACCTCAAAAGTC
AGACAATTACTAGACACTACTGACATCCAAGGAGAAGAATTTGCCAAAGT
TATAGTACAAAAATTGAAAGATAACAAACAAATGGGTCTTCAGCCTTACC
CGGAAATACTTGTGGTTTCTAGATCACCATCTTTAAATTTACTTCAAAAT
AAAAGCATGTAAGGTACC

Amino Acid Sequence

MDYKDDDDKMNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWR
VQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFL
GIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPP
LLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTII
YMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYS
VSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELE
PVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGP
QEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGN
HSWDSTISGSQRAAFCDHKTAPCSSAIINPLSTAGNSERLQPGIAQQWIQ
SKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLL
DTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8569
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 62289 Daltons