cDNA Clone VPS15

• Plasmid: pGEX6P-2-VPS15 1-350
• Parent Plasmid: pGEX6P2
• Expressed: GST-VPS15 1-350
• Genbank: NP_055417
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCT
TTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAA
GCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAA
GGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTT
AACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCTG
CACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAA
GCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCAT
CAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCC
AGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGG
GACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTCT
AACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGG
CAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATT
GCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATA
TATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAA
CAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTG
ATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCAACT
TTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAAA
TTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAG
CCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAA
TGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGT
TTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGG
AAGGATTTGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMGNQLAGI
APSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQ
DPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRD
NLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTS
WNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMF
ATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPL
FDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYL
KQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8563
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST tag for bacterial expression.; ; Estimated MW of expressed protein : 68394 Daltons