cDNA Clone TANK

• Plasmid: pEBG6P-TANK
• Parent Plasmid: pEBG6P
• Expressed: GST-TANK
• Genbank: AAH67779.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATAAAAACATTGGCGAGCAACTCAATAAAGCGTATGAAGC
CTTCCGGCAGGCATGCATGGATAGAGATTCTGCAGTAAAAGAATTACAGC
AAAAGACTGAGAACTATGAGCAGAGAATACGTGAACAACAGGAACAGCTG
TCACTTCAACAGACTATTATTGACAAGCTAAAATCTCAGTTACTTCTTGT
GAATTCCACTCAAGATAACAATTATGGCTGTGTTCCTCTGCTTGAAGACA
GTGAAACAAGAAAGAATAATTTGACTCTTGATCAGCCACAAGATAAAGTG
ATTTCAGGAATAGCAAGAGAAAAACTACCAAAGGTAAGAAGACAAGAGGT
TTCTTCTCCTAGAAAAGAAACTTCAGCAAGGAGTCTTGGCAGTCCTTTGC
TCCATGAAAGGGGTAATATAGAGAAGACTTTCTGGGATCTGAAAGAAGAA
TTTCATAAAATATGCATGCTAGCAAAAGCACAGAAAGACCACTTAAGCAA
ACTTAATATACCAGACACTGCAACTGAAACACAGTGCTCTGTGCCTATAC
AGTGTACGGATAAAACAGATAAACAAGAAGCGCTGTTTAAGCCTCAGGCT
AAAGATGATATAAATAGAGGTGCACCATCCATCACATCTGTCACACCAAG
AGGACTGTGCAGAGATGAGGAAGACACCTCTTTTGAATCACTTTCTAAAT
TCAATGTCAAGTTTCCACCTATGGACAATGACTCAACTTTCTTACATAGC
ACTCCAGAGAGACCCGGCATCCTTAGTCCTGCCACGTCTGAGGTAGTGTG
CCAAGAGAAATTTAATATGGAGTTCAGAGACAACCCAGGGAACTTTGTTA
AAACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAGCTTCA
GCTATACAAAACCTTAAAACAACTGACAAAACAAAGCCCTCAAATCTCGT
AAACACTTGTATCAGGACAACTCTGGATAGAGCTGCGTGTTTGCCACCTG
GAGACCATAATGCATTATATGTAAATAGCTTCCCACTTCTGGACCCATCT
GATGCACCTTTTCCCTCACTCGATTCCCCGGGAAAAGCAATCCGAGGACC
ACAGCAGCCCATTTGGAAGCCCTTTCCTAATCAAGACAGTGACTCGGTGG
TACTAAGTGGCACAGACTCAGAACTGCATATACCTCGAGTATGTGAATTC
TGTCAAGCAGTTTTCCCACCATCCATTACATCCAGGGGGGATTTCCTTCG
GCATCTTAATTCACACTTCAATGGAGAGACTTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMDKNIGEQLNKAY
EAFRQACMDRDSAVKELQQKTENYEQRIREQQEQLSLQQTIIDKLKSQLL
LVNSTQDNNYGCVPLLEDSETRKNNLTLDQPQDKVISGIAREKLPKVRRQ
EVSSPRKETSARSLGSPLLHERGNIEKTFWDLKEEFHKICMLAKAQKDHL
SKLNIPDTATETQCSVPIQCTDKTDKQEALFKPQAKDDINRGAPSITSVT
PRGLCRDEEDTSFESLSKFNVKFPPMDNDSTFLHSTPERPGILSPATSEV
VCQEKFNMEFRDNPGNFVKTEETLFEIQGIDPIASAIQNLKTTDKTKPSN
LVNTCIRTTLDRAACLPPGDHNALYVNSFPLLDPSDAPFPSLDSPGKAIR
GPQQPIWKPFPNQDSDSVVLSGTDSELHIPRVCEFCQAVFPPSITSRGDF
LRHLNSHFNGET*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8535
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 75259 Daltons