cDNA Clone PPP4R2

• Plasmid: pCMV-FLAG PPP4R2 C183A
• Parent Plasmid: pCMV5
• Expressed: FLAG-PPP4R2 C183A
• Genbank: NP_062827
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTGCCAGGCGCC
ATGTTGGAGGGCTGGTGGTAGCGGCTTGGGGAGGTGCTCACTCTGTCGGT
CTTGCTCTCTCGCACGCTTCCCCCGGCTCCCTTCGTTTCCCCCCCCCGGT
CGCCTGCGTGCCGGAGTGTGTGCGAGGGAGGGGGAGGGCGTCGGGGGGGT
GGGGGGAGGCGTTCCGGTCCCCAAAAGACCCGCGGAGGGAGGCGGAGGCT
GTGAGGGACTCCGGGAAGCCATGGACGTCGAGAGGCTCCAGGAGGCGCTG
AAAGATTTTGAGAAGAGGGGGAAAAAGGAAGTTTGTCCTGTCCTGGATCA
GTTTCTTTGTCATGTAGCCAAGACTGGAGAAACAATGATTCAGTGGTCCC
AATTTAAAGGCTATTTTATTTTCAAACTGGAGAAAGTGATGGATGATTTC
AGAACTTCAGCTCCTGAGCCAAGAGGTCCTCCCAACCCTAATGTCGAATA
TATTCCCTTTGATGAAATGAAGGAAAGAATACTGAAAATTGTCACTGGAT
TTAATGGTATCCCTTTTACTATTCAGCGACTAGCTGAATTGTTAACAGAT
CCAAGGAGAAACTATACAGGAACAGACAAATTTCTCAGAGGAGTAGAAAA
GAACGTGATGGTTGTTAGCTGTGTTTATCCTTCTTCAGAGAGAAACAATT
CCAATAGTTTAAATCGAATGAATGGTGTGATGTTTCCTGGAAATGCACCA
AGCTATACTGAGAGGTCTAATATAAATGGGCCTGGGACACCCAGGCCACG
TAATCGACCAAAGGTTTCTCTGTCAGCCCCCATGACAACAAATGGGTGGC
CTGAGAGCACAGACAGCAAAGAGGCAAATTTGCAGCAAAATGAGGAGAAA
ACTCACAGTGACTCTTCGACATCTGAATCAGAAGTTTCCTCAGTGAGCCC
TTTGAGAAATAAACATCCAGATGAAGATGCTGTGGAAGCTGAGGGGCATG
AGGTAAAAAGACTCAGGTTTGACAAAGAAGGTGAAGTCAGAGAAACAGCC
AGTCAAACGACTTCCAGCGAAATTTCTTCAGTTATGGTAGGAGAAACAGA
AGCATCATCTTCATCTCAGGATAAAGACAAAGATAGCCGTTGTACCCGGC
AGCACTGTACAGAAGAGGATGAAGAAGAGGATGAAGAGGAAGAAGAAGAG
TCTTTTATGACATCAAGAGAAATGATCCCAGAAAGAAAAAATCAAGAAAA
AGAATCTGATGATGCCTTAACTGTGAATGAAGAGACTTCTGAAGAAAATA
ATCAAATGGAGGAATCTGATGTGTCTCAAGCTGAGAAAGATTTGCTACAT
TCTGAAGGTAGTGAAAACGAAGGCCCTGAAAGTAAGTGGTTCTTCTGAGT
CGAC

Amino Acid Sequence

MDYKDDDDKCQAPCWRAGGSGLGRCSLCRSCSLARFPRLPSFPPPGRLRA
GVCAREGEGVGGVGGGVPVPKRPAEGGGGCEGLREAMDVERLQEALKDFE
KRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSA
PEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLAELLTDPRRN
YTGTDKFLRGVEKNVMVVSCVYPSSERNNSNSLNRMNGVMFPGNAPSYTE
RSNINGPGTPRPRNRPKVSLSAPMTTNGWPESTDSKEANLQQNEEKTHSD
SSTSESEVSSVSPLRNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTT
SSEISSVMVGETEASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMT
SREMIPERKNQEKESDDALTVNEETSEENNQMEESDVSQAEKDLLHSEGS
ENEGPESKWFF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8513
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG tag for mammalian expression.; ; Estimated MW of expressed protein : 51479 Daltons