cDNA Clone SYK

• Plasmid: pGEX6P-1-SYK
• Parent Plasmid: pGEX6P1
• Expressed: GST-SYK
• Genbank: AAH01645.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTT
CTTTTTCGGCAACATCACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGG
GGGGCATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCAACTACCTG
GGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAGGCACACCACTACAC
CATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGACCC
ATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGC
CTGGTCTGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCC
CAAGACTGGGCCCTTTGAGGATTTGAAGGAAAACCTCATCAGGGAATATG
TGAAGCAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGGCCATCATC
AGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACCACAGCCCATGAAAA
AATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAATTG
TCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGAC
AACAACGGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCTGCA
CTATCGCATCGACAAAGACAAGACAGGGAAGCTCTCCATCCCCGAGGGAA
AGAAGTTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTTATAAAGCA
GATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAAAAAATCGGCACACA
GGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATCCTG
CGACTTGGTCAGCGGGTGGAATAATCTCAAGAATCAAATCATACTCCTTC
CCAAAGCCTGGCCACAGAAAGTCCTCCCCTGCCCAAGGGAACCGGCAAGA
GAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCACCCTGGGCTG
CAGACAAAGGCCCCCAGAGAGAAGCCCTACCCATGGACACAGAGGTGTAC
GAGAGCCCCTACGCGGACCCTGAGGAGATCAGGCCCAAGGAGGTTTACCT
GGACCGAAAGCTGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGTAATT
TTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACC
GTGGCTGTGAAAATACTGAAAAACGAGGCCAATGACCCCGCTCTTAAAGA
TGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACAACCCGTACA
TCGTGCGCATGATCGGGATATGCGAGGCCGAGTCCTGGATGCTAGTTATG
GAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACA
TGTCAAGGATAAGAACATCATAGAACTGGTTCATCAGGTTTCCATGGGCA
TGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGA
AATGTGTTGCTAGTTACCCAACATTATGCCAAGATCAGTGATTTCGGACT
CTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCCAGACCCATG
GAAAGTGGCCTGTCAAGTGGTACGCTCCGGAATGCATCAACTACTACAAG
TTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGC
ATTCTCCTATGGGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAAGTCA
CCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCA
AGAGAGATGTACGATCTCATGAATCTGTGCTGGACATACGATGTGGAAAA
CAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATTACTACTATG
ACGTGGTGAACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASSGMADSANHLPFFFGN
ITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERE
LNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGP
FEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWF
HGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRID
KDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVN
FGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVS
FNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKL
LTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLA
EANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDK
NIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKAL
RADENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYG
QKPYRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGF
AAVELRLRNYYYDVVN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8496
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; Estimated MW of expressed protein : 98887 Daltons