cDNA Clone PYGM (mouse)

• Plasmid: pGEX6P-2-PYGM
• Parent Plasmid: pGEX6P2
• Expressed: GST-PYGM
• Genbank: AAD30476
• Tag: GST
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGTCCAGGCCTCTTTCAGACCAGGATAAGAGAAAGCAAAT
CAGCGTTCGTGGCTTAGCCGGAGTGGAAAATGTGTCGGAGCTAAAAAAGA
ACTTCAACCGCCACCTGCATTTCACCTTGGTCAAGGATCGCAATGTGGCT
ACTCCGAGAGATTACTATTTTGCACTGGCCCACACTGTCCGGGACCACCT
CGTGGGACGCTGGATCCGCACACAGCAGCATTACTACGAAAAGGACCCCA
AGAGGATCTACTACCTGTCTTTGGAGTTCTACATGGGCCGGACACTGCAG
AACACCATGGTGAACCTGGCCTTGGAGAACGCCTGTGATGAGGCCACTTA
CCAGCTGGGCTTGGACATGGAGGAGTTGGAGGAGATCGAGGAGGATGCAG
GGTTGGGCAATGGGGGCCTGGGACGCCTGGCAGCTTGCTTTTTGGACTCC
ATGGCTACCCTGGGGCTCGCTGCCTATGGCTATGGGATCCGCTATGAGTT
TGGGATTTTTAATCAGAAGATCTGTGGGGGCTGGCAGATGGAAGAAGCTG
ATGACTGGCTTCGCTATGGCAACCCCTGGGAGAAGGCCCGCCCAGAGTTC
ACGCTGCCTGTGCATTTCTATGGCCGAGTGGAGCACACCAGCCAGGGTGC
CAAGTGGGTGGACACACAGGTAGTGCTGGCTATGCCCTATGACACTCCTG
TGCCCGGCTATCGGAACAACGTCGTCAACACCATGCGCCTCTGGTCGGCC
AAGGCACCTAACGACTTCAACCTCAAGGACTTCAATGTTGGCGGCTACAT
CCAGGCTGTGCTGGACCGAAACCTGGCTGAGAACATCTCGCGTGTTTTGT
ACCCCAATGATAATTTCTTTGAAGGGAAGGAGCTGCGGCTGAAGCAGGAG
TACTTTGTGGTGGCTGCCACCCTCCAGGACATCATCCGGCGTTTCAAGTC
CTCCAAGTTTGGCAGCCGTGATCCTGTGCGCACAAACTTCGATGCCTTCC
CTGATAAGGTGGCCATCCAGCTCAATGACACCCACCCCTCTTTGGCCATC
CCAGAGCTGATGAGGATTCTGGTGGACCTGGAACGACTGGACTGGGACAA
GGCCTGGGACGTAACAGTGAAGACCTGTGCCTACACCAACCACACGGTGC
TGCCTGAAGCCCTGGAGCGCTGGCCAGTGCACCTCATGGAGACACTGCTG
CCCCGCCACTTGCAGATCATTTACGAGATCAACCAGCGTTTCCTTAACCG
GGTGGCGGCTGCATTCCCTGGGGACGTAGATAGGCTGCGGCGCATGTCAC
TCGTGGAGGAAGGAGCAGTGAAACGCATCAACATGGCACACCTGTGCATT
GCTGGCTCGCACGCTGTCAACGGCGTGGCTCGCATCCATTCGGAGATCCT
CAAGAAAACCATCTTCAAGGACTTTTATGAGCTGGAGCCTCACAAGTTCC
AGAACAAGACCAATGGCATCACTCCTCGGCGCTGGCTGGTTCTGTGTAAC
CCTGGGCTGGCAGAGGTCATTGCTGAGCGCATTGGTGAGGACTACATCTC
AGACCTGGACCAGCTGCGCAAGCTGCTCTCCTATGTGGATGATGAAGCCT
TTATCCGTGATGTGGCCAAAGTGAAACAGGAAAACAAGTTGAAGTTCTCT
GCATACCTCGAGAGGGAATACAAAGTCCACATCAACCCCAACTCGCTCTT
CGACGTCCAGGTGAAAAGGATTCATGAGTACAAGCGGCAGCTCCTCAACT
GCCTGCACATCATCACCTTGTATAACCGCATCAAAAGGGAGCCCAATAGG
TTTATGGTGCCGAGGACTATCATGATTGGAGGCAAGGCTGCACCCGGGTA
CCACATGGCTAAGATGATCATCAAGCTTATCACTGCCATTGGGGATGTGG
TCAACCACGACCCTGCGGTAGGAGACCGCCTCCGAGTGATCTTCCTGGAG
AACTACCGAGTCTCCCTTGCTGAGAAAGTTATTCCTGCCGCCGACCTCTC
AGAGCAGATCTCCACCGCGGGCACTGAGGCCTCGGGCACTGGCAACATGA
AGTTCATGCTCAATGGGGCTCTGACCATCGGCACCATGGACGGTGCCAAC
GTGGAGATGGCAGAGGAGGCGGGGGAGGAGAACTTCTTCATCTTCGGCAT
GCGAGTGGAGGACGTGGAAAGGCTGGACCAGAGAGGGTACAATGCCCAGG
AGTACTACGACCGAATTCCTGAGCTTCGGCAGATCATCGAACAGCTAAGC
AGCGGCTTCTTCTCCCCCAAGCAGCCTGACCTGTTCAAGGACATTGTCAA
TATGCTCATGCACCATGACCGGTTTAAAGTCTTTGCAGATTATGAGGAAT
ACATTAAATGCCAGGACAAAGTCAGTGAGTTGTACAAGAACCCAAGAGAG
TGGACACGGATGGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAG
TGACCGCACCATTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTT
CTCGCCAGCGCCTGCCAGCCCCAGATGAGAAGATCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMSRPLSDQ
DKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATPRDYYFALAH
TVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENA
CDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGY
GIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVE
HTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDF
NVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDI
IRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLE
RLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEIN
QRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVAR
IHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERI
GEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHI
NPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGG
KAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVI
PAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEEN
FFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDL
FKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIAT
SGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKI*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-2-PYGM57.19 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU8487
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 125085 Daltons
• Low Temp: No