cDNA Clone PYGM (mouse)

• Plasmid: pFBHTb PYGM
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PYGM
• Genbank: AAD30476
• Tag: 6His
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGTCCAGGCCTCTTTCAGACCAGGATAAGAGAAAGCAAAT
CAGCGTTCGTGGCTTAGCCGGAGTGGAAAATGTGTCGGAGCTAAAAAAGA
ACTTCAACCGCCACCTGCATTTCACCTTGGTCAAGGATCGCAATGTGGCT
ACTCCGAGAGATTACTATTTTGCACTGGCCCACACTGTCCGGGACCACCT
CGTGGGACGCTGGATCCGCACACAGCAGCATTACTACGAAAAGGACCCCA
AGAGGATCTACTACCTGTCTTTGGAGTTCTACATGGGCCGGACACTGCAG
AACACCATGGTGAACCTGGCCTTGGAGAACGCCTGTGATGAGGCCACTTA
CCAGCTGGGCTTGGACATGGAGGAGTTGGAGGAGATCGAGGAGGATGCAG
GGTTGGGCAATGGGGGCCTGGGACGCCTGGCAGCTTGCTTTTTGGACTCC
ATGGCTACCCTGGGGCTCGCTGCCTATGGCTATGGGATCCGCTATGAGTT
TGGGATTTTTAATCAGAAGATCTGTGGGGGCTGGCAGATGGAAGAAGCTG
ATGACTGGCTTCGCTATGGCAACCCCTGGGAGAAGGCCCGCCCAGAGTTC
ACGCTGCCTGTGCATTTCTATGGCCGAGTGGAGCACACCAGCCAGGGTGC
CAAGTGGGTGGACACACAGGTAGTGCTGGCTATGCCCTATGACACTCCTG
TGCCCGGCTATCGGAACAACGTCGTCAACACCATGCGCCTCTGGTCGGCC
AAGGCACCTAACGACTTCAACCTCAAGGACTTCAATGTTGGCGGCTACAT
CCAGGCTGTGCTGGACCGAAACCTGGCTGAGAACATCTCGCGTGTTTTGT
ACCCCAATGATAATTTCTTTGAAGGGAAGGAGCTGCGGCTGAAGCAGGAG
TACTTTGTGGTGGCTGCCACCCTCCAGGACATCATCCGGCGTTTCAAGTC
CTCCAAGTTTGGCAGCCGTGATCCTGTGCGCACAAACTTCGATGCCTTCC
CTGATAAGGTGGCCATCCAGCTCAATGACACCCACCCCTCTTTGGCCATC
CCAGAGCTGATGAGGATTCTGGTGGACCTGGAACGACTGGACTGGGACAA
GGCCTGGGACGTAACAGTGAAGACCTGTGCCTACACCAACCACACGGTGC
TGCCTGAAGCCCTGGAGCGCTGGCCAGTGCACCTCATGGAGACACTGCTG
CCCCGCCACTTGCAGATCATTTACGAGATCAACCAGCGTTTCCTTAACCG
GGTGGCGGCTGCATTCCCTGGGGACGTAGATAGGCTGCGGCGCATGTCAC
TCGTGGAGGAAGGAGCAGTGAAACGCATCAACATGGCACACCTGTGCATT
GCTGGCTCGCACGCTGTCAACGGCGTGGCTCGCATCCATTCGGAGATCCT
CAAGAAAACCATCTTCAAGGACTTTTATGAGCTGGAGCCTCACAAGTTCC
AGAACAAGACCAATGGCATCACTCCTCGGCGCTGGCTGGTTCTGTGTAAC
CCTGGGCTGGCAGAGGTCATTGCTGAGCGCATTGGTGAGGACTACATCTC
AGACCTGGACCAGCTGCGCAAGCTGCTCTCCTATGTGGATGATGAAGCCT
TTATCCGTGATGTGGCCAAAGTGAAACAGGAAAACAAGTTGAAGTTCTCT
GCATACCTCGAGAGGGAATACAAAGTCCACATCAACCCCAACTCGCTCTT
CGACGTCCAGGTGAAAAGGATTCATGAGTACAAGCGGCAGCTCCTCAACT
GCCTGCACATCATCACCTTGTATAACCGCATCAAAAGGGAGCCCAATAGG
TTTATGGTGCCGAGGACTATCATGATTGGAGGCAAGGCTGCACCCGGGTA
CCACATGGCTAAGATGATCATCAAGCTTATCACTGCCATTGGGGATGTGG
TCAACCACGACCCTGCGGTAGGAGACCGCCTCCGAGTGATCTTCCTGGAG
AACTACCGAGTCTCCCTTGCTGAGAAAGTTATTCCTGCCGCCGACCTCTC
AGAGCAGATCTCCACCGCGGGCACTGAGGCCTCGGGCACTGGCAACATGA
AGTTCATGCTCAATGGGGCTCTGACCATCGGCACCATGGACGGTGCCAAC
GTGGAGATGGCAGAGGAGGCGGGGGAGGAGAACTTCTTCATCTTCGGCAT
GCGAGTGGAGGACGTGGAAAGGCTGGACCAGAGAGGGTACAATGCCCAGG
AGTACTACGACCGAATTCCTGAGCTTCGGCAGATCATCGAACAGCTAAGC
AGCGGCTTCTTCTCCCCCAAGCAGCCTGACCTGTTCAAGGACATTGTCAA
TATGCTCATGCACCATGACCGGTTTAAAGTCTTTGCAGATTATGAGGAAT
ACATTAAATGCCAGGACAAAGTCAGTGAGTTGTACAAGAACCCAAGAGAG
TGGACACGGATGGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAG
TGACCGCACCATTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTT
CTCGCCAGCGCCTGCCAGCCCCAGATGAGAAGATCTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMSRPLSD
QDKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATPRDYYFALA
HTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALEN
ACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYG
YGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRV
EHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKD
FNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQD
IIRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDL
ERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEI
NQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVA
RIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAER
IGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVH
INPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIG
GKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKV
IPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEE
NFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPD
LFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIA
TSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8481
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 102094 Daltons