cDNA Clone RAD5

• Plasmid: pGEX6P-2-RAD5
• Parent Plasmid: pGEX6P2
• Expressed: GST-RAD5
• Genbank: CAB45724.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGCCAGCACCCTCAGAGGTTCGACTCGCCGTCCGGGA
AGCCATTCATGCCCTCTCGTCTTCGGAGGATGGCGGCCACATCTTCTGCA
CCCTGGAGTCCCTGAAGCGGTATCTCGGTGAGATGGAGCCTCCAGCGCTC
CCGAGGGAGAAGGAGGAGTTTGCCTCGGCCCACTTCTCGCCTGTCCTCAG
ATGTCTTGCCAGCAGGCTGAGCCCAGCCTGGCTGGAGCTGCTGCCCCATG
GCCGCCTGGAGGAGCTGTGGGCCAGCTTCTTCCTGGAGGGCCCGGCGGAC
CAAGCCTTCCTGGTGTTGATGGAGACCATCGAGGGTGCTGCGGGCCCCAG
CTTCCGGCTGATGAAGATGGCGCGGCTGCTGGCCAGATTCCTGCGCGAGG
GCCGGCTGGCAGTGCTGATGGAGGCGCAGTGTCGGCAGCAGACGCGGCCC
GGCTTCATCCTGCTCCGGGAGACGCTGCTGGGCAAGGTGGTGGCCCTGCC
CGATCACCTGGGCAACCGCCTGCAGCAGGAGAACTTGGCCGAGTTCTTCC
CCCAGAACTACTTCCGCCTGCTCGGCGAGGAGGTCGTCCGGGTGCTGCAG
GCGGTTGTGGACTCTCTCCAAGGTGGCCTGGATTCCTCCGTGTCCTTCGT
GTCTCAGGTCCTTGGGAAAGCCTGTGTCCACGGGAGGCAGCAGGAGATCC
TGGGCGTGCTGGTACCCCGGCTGGCAGCGCTCACCCAGGGCAGCTACCTG
CACCAGCGCGTCTGCTGGCGCCTGGTGGAGCAAGTGCCGGACCGGGCCAT
GGAGGCTGTGCTGACCGGGCTGGTGGAGGCCGCACTGGGGCCTGAGGTCC
TTTCGAGACTGCTGGGGAACCTGGTGGTGAAGAACAAGAAGGCCCAGTTT
GTGATGACCCAGAAGCTTCTGTTCTTACAGTCCCGGCTCACGACGCCCAT
GCTGCAGAGCCTGCTGGGCCATCTGGCCATGGACAGCCAGCGGCGCCCGC
TCCTGCTGCAGGTGCTGAAGGAGCTGTTGGAGACGTGGGGCAGCAGCAGT
GCCATCCGCCACACTCCCCTGCCGCAGCAGCGCCACGTCAGCAAGGCTGT
CCTCATCTGCCTGGCGCAACTCGGGGAGCCGGAACTGCGGGACAGCCGGG
ATGAACTGCTGGCCAGCATGATGGCGGGCGTGAAGTGCCGCCTGGACAGT
AGCCTGCCCCCCGTGCGACGCCTGGGCATGATCGTGGCAGAGGTCGTTAG
TGCCCGGATCCACCCCGAGGGGCCTCCCCTGAAATTCCAGTACGAAGAGG
ATGAACTGAGCCTCGAGCTGCTGGCCTTGGCCTCCCCCCAGCCTGCGGGT
GACGGCGCCTCGGAGGCGGGCACGTCCCTCGTTCCAGCCACGGCAGAGCC
CCCTGCAGAGACCCCCGCAGAGATCGTGGATGGCGGCGTCCCCCAAGCAC
AGCTGGCGGGCTCTGACTCGGACCTGGACAGCGATGATGAGTTTGTCCCC
TACGACATGTCGGGGGACAGAGAGCTGAAGAGCAGCAAGGCTCCTGCCTA
CGTCCGGGACTGCGTGGAAGCCCTGACCACGTCTGAGGACATAGAGCGCT
GGGAGGCAGCCCTGCGGGCCCTTGAGGGCCTGGTCTACAGGAGCCCCACA
GCCACTCGGGAGGTGAGCGTGGAGCTGGCCAAGGTGCTTCTGCATCTGGA
GGAGAAGACCTGTGTGGTGGGATTTGCAGGGCTGCGCCAGAGAGCCCTGG
TGGCCGTCACGGTCACAGACCCGGCCCCGGTGGCCGACTATCTGACCTCA
CAGTTCTATGCCCTCAACTACAGCCTCCGGCAGCGCATGGACATCCTGGA
TGTGCTGACTCTGGCTGCCCAGGAGCTGTCTAGGCCTGGGTGCCTCGGGA
GGACTCCCCAACCTGGCTCCCCAAGTCCCAACACCCCGTGCCTGCCAGAG
GCAGCCGTCTCTCAGCCTGGCAGTGCCGTGGCGTCTGACTGGCGGGTGGT
GGTGGAGGAGCGGATCAGAAGCAAGACCCAGCGGCTCTCCAAGGGTGGCC
CGAGGCAGGGCCCGGCAGGCAGCCCCAGCAGATTCAACTCCGTGGCCGGC
CACTTCTTCTTCCCCCTCCTTCAGCGCTTTGACAGGCCTCTGGTGACCTT
CGACCTCTTGGGAGAAGACCAGCTGGTTCTCGGAAGGCTGGCGCACACCT
TAGGGGCCCTGATGTGCCTGGCTGTTAACACCACGGTGGCTGTGGCCATG
GGCAAGGCCCTGCTGGAATTCGTGTGGGCCCTTCGCTTCCACATCGATGC
CTACGTGCGCCAGGGGCTGTTGTCGGCCGTCTCCTCCGTCCTGCTCAGCC
TGCCTGCTGCGCGCCTGCTGGAGGACCTGATGGACGAGCTGCTGGAAGCC
CGGTCCTGGCTGGCGGACGTGGCTGAGAAAGACCCGGACGAGGACTGCAG
GACGCTGGCACTGAGGGCCCTGCTGCTTCTGCAGAGACTCAAGAACAGGC
TCCTCCCACCCGCGTCTCCCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMEPAPSEV
RLAVREAIHALSSSEDGGHIFCTLESLKRYLGEMEPPALPREKEEFASAH
FSPVLRCLASRLSPAWLELLPHGRLEELWASFFLEGPADQAFLVLMETIE
GAAGPSFRLMKMARLLARFLREGRLAVLMEAQCRQQTRPGFILLRETLLG
KVVALPDHLGNRLQQENLAEFFPQNYFRLLGEEVVRVLQAVVDSLQGGLD
SSVSFVSQVLGKACVHGRQQEILGVLVPRLAALTQGSYLHQRVCWRLVEQ
VPDRAMEAVLTGLVEAALGPEVLSRLLGNLVVKNKKAQFVMTQKLLFLQS
RLTTPMLQSLLGHLAMDSQRRPLLLQVLKELLETWGSSSAIRHTPLPQQR
HVSKAVLICLAQLGEPELRDSRDELLASMMAGVKCRLDSSLPPVRRLGMI
VAEVVSARIHPEGPPLKFQYEEDELSLELLALASPQPAGDGASEAGTSLV
PATAEPPAETPAEIVDGGVPQAQLAGSDSDLDSDDEFVPYDMSGDRELKS
SKAPAYVRDCVEALTTSEDIERWEAALRALEGLVYRSPTATREVSVELAK
VLLHLEEKTCVVGFAGLRQRALVAVTVTDPAPVADYLTSQFYALNYSLRQ
RMDILDVLTLAAQELSRPGCLGRTPQPGSPSPNTPCLPEAAVSQPGSAVA
SDWRVVVEERIRSKTQRLSKGGPRQGPAGSPSRFNSVAGHFFFPLLQRFD
RPLVTFDLLGEDQLVLGRLAHTLGALMCLAVNTTVAVAMGKALLEFVWAL
RFHIDAYVRQGLLSAVSSVLLSLPAARLLEDLMDELLEARSWLADVAEKD
PDEDCRTLALRALLLLQRLKNRLLPPASP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8341
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 119572 Daltons