cDNA Clone 14-3-3 gamma

• Synonyms: YWHAG
• Plasmid: pGEX6P-1-14-3-3 gamma
• Parent Plasmid: pGEX6P1
• Expressed: GST-14-3-3 gamma
• Genbank: AAH20963.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTGGACCGCGAGCAACTGGTGCAGAAAGCCCGGCTGGCCGA
GCAGGCGGAGCGCTACGACGACATGGCCGCGGCCATGAAGAACGTGACAG
AGCTGAATGAGCCACTGTCGAATGAGGAACGAAACCTTCTGTCTGTGGCC
TACAAGAACGTTGTGGGGGCACGCCGCTCTTCCTGGAGGGTCATCAGTAG
CATTGAGCAGAAGACATCTGCAGACGGCAATGAGAAGAAGATTGAGATGG
TCCGTGCGTACCGGGAGAAGATAGAGAAGGAGTTGGAGGCTGTGTGCCAG
GATGTGCTGAGCCTGCTGGATAACTACCTGATCAAGAATTGCAGCGAGAC
CCAGTACGAGAGCAAAGTGTTCTACCTGAAGATGAAAGGGGACTACTACC
GCTACCTGGCTGAAGTGGCCACCGGAGAGAAAAGGGCGACGGTGGTGGAG
TCCTCTGAGAAGGCCTACAGCGAAGCCCACGAGATCAGCAAAGAGCACAT
GCAGCCCACCCACCCCATCCGATTAGGCCTGGCTCTTAACTACTCCGTCT
TCTACTATGAGATCCAGAACGCCCCAGAGCAAGCGTGCCACTTGGCCAAG
ACCGCGTTCGACGACGCCATCGCCGAGCTTGACACCCTCAACGAGGACTC
CTACAAGGACTCCACGCTCATCATGCAGCTCCTCCGCGACAACCTCACGC
TCTGGACGAGCGACCAGCAGGACGACGATGGCGGCGAAGGCAACAATTAA
GCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVDREQLVQKARLAEQAER
YDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIEQK
TSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYES
KVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTH
PIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDS
TLIMQLLRDNLTLWTSDQQDDDGGEGNN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8296
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; Estimated MW of expressed protein : 55124 Daltons