cDNA Clone 14-3-3 eta

• Synonyms: YWHAH
• Plasmid: pET28a 14-3-3 eta
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 eta
• Genbank: NP_003396.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGGGGACCGGGAGCAGCTGCTGCAGCGGGCGCGGCTGGCCGA
GCAGGCGGAGCGCTACGACGACATGGCCTCCGCTATGAAGGCGGTGACAG
AGCTGAATGAACCTCTCTCCAATGAAGATCGAAATCTCCTCTCTGTGGCC
TACAAGAATGTGGTTGGTGCCAGGCGATCTTCCTGGAGGGTCATTAGCAG
CATTGAGCAGAAAACCATGGCTGATGGAAACGAAAAGAAATTGGAGAAAG
TTAAAGCTTACCGGGAGAAGATTGAGAAGGAGCTGGAGACAGTTTGCAAT
GATGTCCTGTCTCTGCTTGACAAGTTCCTGATCAAGAACTGCAATGATTT
CCAGTATGAGAGCAAGGTGTTTTACCTGAAAATGAAGGGTGATTACTACC
GCTACTTAGCAGAGGTCGCTTCTGGGGAGAAGAAAAACAGTGTGGTCGAA
GCTTCTGAAGCTGCCTACAAGGAAGCCTTTGAAATCAGCAAAGAGCAGAT
GCAACCCACGCATCCCATCCGGCTGGGCCTGGCCCTCAACTTCTCCGTGT
TCTACTATGAGATCCAGAATGCACCTGAGCAAGCCTGCCTCTTAGCCAAA
CAAGCCTTCGATGATGCCATAGCTGAGCTGGACACACTAAACGAGGATTC
CTATAAGGACTCCACGCTGATCATGCAGTTGCTGCGAGACAACCTCACCC
TCTGGACGAGCGACCAGCAGGATGAAGAAGCAGGAGAAGGCAACTGAGCG
GCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMGDREQLLQRARLAEQ
AERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSI
EQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQ
YESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQ
PTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSY
KDSTLIMQLLRDNLTLWTSDQQDEEAGEGN*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8263
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.