cDNA Clone PP4R3

• Plasmid: pEGFP-C1-PP4R3a
• Parent Plasmid: pEGFPC1
• Expressed: GFP-PP4R3
• Genbank: AAH72409
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGA
GGACCGGCAGTGGGACGACCGGGGCACCGGGCATGTGTCGTCTGGCTACG
TGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGACGGT
TCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACA
ACAGGACACTCTGATTGTGTGGTCTGAAGCAGAAAATTATGACTTGGCCC
TTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTTGGGAGAAAATATGT
CAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGA
TGAATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAAT
TGCCATCTTGTGAATTAAGTCGCCTTGAAGAAATTGCAGAACTTGTGGCA
TCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCACTAGA
AAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAG
ATTTGGAAAATATTGAAGGACTGCACCACTTGTATGAAATTATCAAAGGC
ATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTCTCTGAAGA
ATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCAC
AACCACGAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAA
GTGATTCCCATATCAGATCCTGAGCTGAAACAAAAAATTCATCAGACATA
CAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTCTTTG
AAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTA
GAGATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACAGATTTGTT
TGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGG
TTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAA
AACAGAGATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGC
TTTAGAAGTCATCCTTGGCATGGATGATACACAGGTGCGAAGTGCTGCTA
CTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAG
TTTGTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAAC
AGAGCAAAAAATAACCAGCAAGGATATTTTGCTCATCAACCTCATTATAG
AACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTT
ATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGC
CAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTA
TGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCT
AGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATT
GTTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTACATTA
TTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCAT
GCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATT
AAAAGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAAC
CAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGATGAAC
TCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATATAAAATC
ATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGATGTAG
ATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGA
GAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAA
TCACAGATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGT
GGTTTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCT
CCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATAAGTAA
ATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTC
TGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTG
TCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCT
GCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTG
GATCCGTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGA
GGCCTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGA
GGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAAATTTG
ATTCATAAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFMTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDG
SLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKIC
QVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVA
SSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKG
IFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKE
VIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKV
EIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQ
NRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVRE
FVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQL
MGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKP
SKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKH
AFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMN
SAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQR
ERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVS
PSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSL
SSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKG
GLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8242
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GFP for mammalian expression.; Estimated MW of expressed protein : 123592 Daltons