cDNA Clone OSTB

• Plasmid: pCMV-FLAG-1 OTSB
• Parent Plasmid: pCMV5 FLAG1
• Genbank: ECOTSAB
• Tag: FLAG
• Species: Escherichia coli

Sequence of Insert

GAATTCATGAGTCGTTTAGTCGTAGTATCTAACCGGATTGCACCACCAGA
CGAGCACGCCGCCAGTGCCGGTGGCCTTGCCGTTGGCATACTGGGGGCAC
TGAAAGCCGCAGGCGGACTGTGGTTTGGCTGGAGTGGTGAAACAGGGAAT
GAGGATCAGCCGCTAAAAAAGGTGAAAAAAGGTAACATTACGTGGGCCTC
TTTTAACCTCAGCGAACAGGACCTTGACGAATACTACAACCAATTCTCCA
ATGCCGTTCTCTGGCCCGCTTTTCATTATCGGCTCGATCTGGTGCAATTT
CAGCGTCCTGCCTGGGACGGCTATCTACGCGTAAATGCGTTGCTGGCAGA
TAAATTACTGCCGCTGTTGCAAGACGATGACATTATCTGGATCCACGATT
ATCACCTGTTGCCATTTGCGCATGAATTACGCAAACGGGGAGTGAATAAT
CGCATTGGTTTCTTTCTGCATATTCCTTTCCCGACACCGGAAATCTTCAA
CGCGCTGCCGACATATGACACCTTGCTTGAACAGCTTTGTGATTATGATT
TGCTGGGTTTCCAGACAGAAAACGATCGTCTGGCGTTCCTGGATTGTCTT
TCTAACCTGACCCGCGTCACGACACGTAGCGCAAAAAGCCATACAGCCTG
GGGCAAAGCATTTCGAACAGAAGTCTACCCGATCGGCATTGAACCGAAAG
AAATAGCCAAACAGGCTGCCGGGCCACTGCCGCCAAAACTGGCGCAACTT
AAAGCGGAACTGAAAAACGTACAAAATATCTTTTCTGTCGAACGGCTGGA
TTATTCCAAAGGTTTGCCAGAGCGTTTTCTCGCCTATGAAGCGTTGCTGG
AAAAATATCCGCAGCATCATGGTAAAATTCGTTATACCCAGATTGCACCA
ACGTCGCGTGGTGATGTGCAAGCCTATCAGGATATTCGTCATCAGCTCGA
AAATGAAGCTGGACGAATTAATGGTAAATACGGGCAATTAGGCTGGACGC
CGCTTTATTATTTGAATCAGCATTTTGACCGTAAATTACTGATGAAAATA
TTCCGCTACTCTGACGTGGGCTTAGTGACGCCACTGCGTGACGGGATGAA
CCTGGTAGCAAAAGAGTATGTTGCTGCTCAGGACCCAGCCAATCCGGGCG
TTCTTGTTCTTTCGCAATTTGCGGGAGCGGCAAACGAGTTAACGTCGGCG
TTAATTGTTAACCCCTACGATCGTGACGAAGTTGCAGCTGCGCTGGATCG
TGCATTGACTATGTCGCTGGCGGAACGTATTTCCCGTCATGCAGAAATGC
TGGACGTTATCGTGAAAAACGATATTAACCACTGGCAGGAGTGCTTCATT
AGCGACCTAAAGCAGATAGTTCCGCGAAGCGCGGAAAGCCAGCAGCGCGA
TAAAGTTGCTACCTTTCCAAAGCTTGCGTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPEFMSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAG
GLWFGWSGETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLW
PAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLP
FAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQ
TENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQ
AAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQ
HHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYL
NQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLS
QFAGAANELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIV
KNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKLA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; Estimated MW of expressed protein : 55252 Daltons