cDNA Clone POLDIP3

• Plasmid: pCMV-HA-2 POLDIP3
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-POLDIP3
• Genbank: AAH49840
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGACATCTCCCTGGACGAACTCATCAGGAAGCGCGG
GGCGGCGGCGAAAGGACGGCTTAATGCCAGACCGGGAGTTGGAGGTGTCC
GATCTCGAGTTGGGATCCAGCAAGGCCTTCTCAGCCAGTCAACACGCACA
GCCACCTTCCAGCAGAGATTTGATGCCCGGCAGAAGATTGGCCTCTCAGA
TGCCCGGCTCAAACTGGGAGTCAAGGATGCCCGGGAGAAGCTTTTGCAGA
AAGATGCCCGATTTCGAATCAAAGGGAAAGTGCAGGATGCCAGAGAGATG
TTGAACTCTCGCAAGCAGCAGACCACGGTGCCCCAGAAGCCCCGCCAGGT
TGCTGATGCCCGGGAGAAGATCAGCTTGAAGAGGAGTTCCCCTGCTGCCT
TCATAAACCCACCCATTGGGACAGTGACCCCTGCTCTGAAGCTCACCAAA
ACCATCCAGGTTCCACAGCAGAAAGCCATGGCACCACTTCATCCCCATCC
TGCCGGAATGAGAATCAATGTTGTCAATAACCACCAGGCCAAACAGAATT
TATATGACCTGGATGAAGATGATGATGGTATAGCTTCCGTTCCTACTAAA
CAGATGAAGTTTGCAGCCTCAGGCGGCTTTCTCCACCACATGGCTGGGCT
AAGCAGTTCCAAGCTTTCCATGTCCAAGGCCCTCCCTCTCACCAAAGTGG
TTCAGAATGATGCATACACAGCTCCTGCTCTCCCTTCCTCTATTCGAACA
AAAGCCTTGACCAACATGTCCCGGACACTGGTGAACAAGGAAGAACCCCC
CAAAGAGCTGCCAGCTGCTGAGCCTGTTCTCAGCCCATTGGAAGGCACCA
AGATGACTGTGAATAATCTGCACCCTCGAGTCACTGAGGAGGACATTGTT
GAGCTTTTCTGTGTGTGTGGGGCCCTCAAGCGAGCTCGACTGGTCCATCC
TGGGGTAGCGGAGGTGGTGTTTGTGAAAAAGGACGATGCCATCACCGCAT
ATAAGAAGTACAACAACCGGTGTCTGGACGGGCAGCCGATGAAGTGCAAC
CTTCACATGAATGGGAATGTTATCACCTCAGACCAGCCCATCCTGCTGCG
GCTGAGTGACAGCCCATCAATGAAAAAGGAGAGCGAGCTGCCTCGCAGGG
TGAACTCTGCCTCCTCCTCCAACCCCCCTGCCGAAGTGGACCCTGACACC
ATCCTGAAGGCACTCTTCAAGTCCTCAGGGGCCTCTGTGACCACGCAGCC
CACAGAATTCAAAATCAAGCTTTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMADISLDELIRKRGAAAKGRLNARPGV
GGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREK
LLQKDARFRIKGKVQDAREMLNSRKQQTTVPQKPRQVADAREKISLKRSS
PAAFINPPIGTVTPALKLTKTIQVPQQKAMAPLHPHPAGMRINVVNNHQA
KQNLYDLDEDDDGIASVPTKQMKFAASGGFLHHMAGLSSSKLSMSKALPL
TKVVQNDAYTAPALPSSIRTKALTNMSRTLVNKEEPPKELPAAEPVLSPL
EGTKMTVNNLHPRVTEEDIVELFCVCGALKRARLVHPGVAEVVFVKKDDA
ITAYKKYNNRCLDGQPMKCNLHMNGNVITSDQPILLRLSDSPSMKKESEL
PRRVNSASSSNPPAEVDPDTILKALFKSSGASVTTQPTEFKIKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8098
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; Estimated MW of expressed protein : 48423 Daltons